{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T09:52:47Z","timestamp":1793699567556,"version":"4.5.1"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,14]],"date-time":"2012-02-14T00:00:00Z","timestamp":1329177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Quorum sensing, a bacterial cell\u2013cell communication process, controls biofilm formation and virulence factor production in <jats:italic>Vibrio cholerae<\/jats:italic>, a human pathogen that causes the disease cholera. The major <jats:italic>V.\u2003cholerae<\/jats:italic> autoinducer is (<jats:italic>S<\/jats:italic>)\u20103\u2010hydroxytridecan\u20104\u2010one (CAI\u20101). A membrane bound two\u2010component sensor histidine kinase called CqsS detects CAI\u20101, and the CqsS\u2003\u2192\u2003LuxU\u2003\u2192\u2003LuxO phosphorelay cascade transduces the information encoded in CAI\u20101 into the cell. Because the CAI\u20101 ligand is known and because the signalling circuit is simple, consisting of only three proteins, this system is ideal for analysing ligand regulation of a sensor histidine kinase. Here we reconstitute the CqsS\u2003\u2192\u2003LuxU\u2003\u2192\u2003LuxO phosphorylation cascade <jats:italic>in vitro<\/jats:italic>. We find that CAI\u20101 inhibits the initial auto\u2010phosphorylation of CqsS whereas subsequent phosphotransfer steps and CqsS phosphatase activity are not CAI\u20101\u2010controlled. CAI\u20101 binding to CqsS causes a conformational change that renders His194 in CqsS inaccessible to the CqsS catalytic domain. CqsS mutants with altered ligand detection specificities are faithfully controlled by their corresponding modified ligands <jats:italic>in vitro<\/jats:italic>. Likewise, pairing of agonists and antagonists allows <jats:italic>in vitro<\/jats:italic> assessment of their opposing activities. Our data are consistent with a two\u2010state model for ligand control of histidine kinases.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.07992.x","type":"journal-article","created":{"date-parts":[[2012,2,2]],"date-time":"2012-02-02T09:54:53Z","timestamp":1328176493000},"page":"1095-1108","source":"Crossref","is-referenced-by-count":55,"title":["Ligand and antagonist driven regulation of the <i>Vibrio cholerae<\/i> quorum\u2010sensing receptor CqsS"],"prefix":"10.1111","volume":"83","author":[{"given":"Yunzhou","family":"Wei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai\u2010Leung","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Cong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie 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