{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:39:49Z","timestamp":1790728789295,"version":"4.1.0"},"reference-count":74,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T00:00:00Z","timestamp":1332201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In <jats:italic>Bacillus anthracis<\/jats:italic> the siderophore petrobactin is vital for iron acquisition and virulence. The petrobactin\u2010binding receptor FpuA is required for these processes. Here additional components of petrobactin reacquisition are described. To identify these proteins, mutants of candidate permease and ATPase genes were generated allowing for characterization of multiple petrobactin ATP\u2010binding cassette (ABC)\u2010import systems. Either of two distinct permeases, FpuB or FatCD, is required for iron acquisition and play redundant roles in petrobactin transport. A mutant strain lacking both permeases, \u0394<jats:italic>fpuB<\/jats:italic>\u0394<jats:italic>fatCD<\/jats:italic>, was incapable of using petrobactin as an iron source and exhibited attenuated virulence in a murine model of inhalational anthrax infection. ATPase mutants were generated in either of the permease mutant backgrounds to identify the ATPase(s) interacting with each individual permease channel. Mutants lacking the FpuB permease and FatE ATPase (\u0394<jats:italic>fpuB<\/jats:italic>\u0394<jats:italic>fatE<\/jats:italic>) and a mutant lacking the distinct ATPases FpuC and FpuD generated in the <jats:italic>\u0394fatCD<\/jats:italic> background (<jats:italic>\u0394fatCD\u0394fpuC\u0394fpuD<\/jats:italic>) displayed phenotypic characteristics of a mutant deficient in petrobactin import. A mutant lacking all three of the identified ATPases (<jats:italic>\u0394fatE\u0394fpuC\u0394fpuD<\/jats:italic>) exhibited the same growth defect in iron\u2010depleted conditions. Taken together, these results provide the first description of the permease and ATPase proteins required for the import of petrobactin in <jats:italic>B.\u2003anthracis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.08028.x","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T09:18:54Z","timestamp":1332235134000},"page":"370-382","source":"Crossref","is-referenced-by-count":22,"title":["Multiple ABC transporters are involved in the acquisition of petrobactin in <i>Bacillus anthracis<\/i>"],"prefix":"10.1111","volume":"84","author":[{"given":"Shandee D.","family":"Dixon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian K.","family":"Janes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Bourgis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul 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