{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:17:46Z","timestamp":1791267466637,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,6,14]],"date-time":"2012-06-14T00:00:00Z","timestamp":1339632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Legionella pneumophila<\/jats:italic>, the causative agent of Legionnaires' disease, survives in macrophages by altering the endocytic pathway of its host cell. To accomplish this, the bacterium utilizes a type IVB secretion system to deliver effector molecules into the host cell cytoplasm. In a previous report, we performed an extensive characterization of the <jats:italic>L.\u2003pneumophila<\/jats:italic> type IVB secretion system that resulted in the identification of a critical five\u2010protein subcomplex that forms the core of the secretion apparatus. Here we describe a second Dot\/Icm protein subassembly composed of the type IV coupling protein DotL, the apparatus proteins DotM and DotN, and the secretion adaptor proteins IcmS and IcmW. In the absence of IcmS or IcmW, DotL becomes destabilized at the transition from the exponential to stationary phases of growth, concurrent with the expression of many secreted substrates. Loss of DotL is dependent on ClpA, a regulator of the cytoplasmic protease ClpP. The resulting decreased levels of DotL in the <jats:italic>icmS<\/jats:italic> and <jats:italic>icmW<\/jats:italic> mutants exacerbates the intracellular defects of these strains and can be partially suppressed by overproduction of DotL. Thus, in addition to their role as chaperones for <jats:italic>Legionella<\/jats:italic> type IV secretion system substrates, IcmS and IcmW perform a second function as part of the Dot\/Icm type IV coupling protein subcomplex.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.08118.x","type":"journal-article","created":{"date-parts":[[2012,6,14]],"date-time":"2012-06-14T06:49:15Z","timestamp":1339656555000},"page":"378-391","source":"Crossref","is-referenced-by-count":64,"title":["Identification of the DotL coupling protein subcomplex of the <i>Legionella<\/i> Dot\/Icm type IV secretion system"],"prefix":"10.1111","volume":"85","author":[{"given":"Carr D.","family":"Vincent","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R.","family":"Friedman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang 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