{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:38:53Z","timestamp":1790221133113,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,7,12]],"date-time":"2012-07-12T00:00:00Z","timestamp":1342051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In <jats:italic>Aspergillus nidulans<\/jats:italic> the global regulatory gene <jats:italic>veA<\/jats:italic> is necessary for the biosynthesis of several secondary metabolites, including the mycotoxin sterigmatocystin (ST). In order to identify additional <jats:italic>veA<\/jats:italic>\u2010dependent genetic elements involved in regulating ST production, we performed a mutagenesis on a deletion <jats:italic>veA<\/jats:italic> (\u0394<jats:italic>veA<\/jats:italic>) strain to obtain revertant mutants (RM) that regained the capability to produce toxin. Genetic analysis and molecular characterization of one of the revertant mutants, RM3, revealed that a point mutation occurred at the coding region of the <jats:italic>rtfA<\/jats:italic> gene, encoding a RNA\u2010pol II transcription elongation factor\u2010like protein, similar to <jats:italic>Saccharomyces cerevisiae<\/jats:italic> Rtf1. The <jats:italic>A.\u2003nidulans rtfA<\/jats:italic> gene product accumulates in nuclei. Deletion of <jats:italic>rtfA<\/jats:italic> gene in a \u0394<jats:italic>veA<\/jats:italic> background restored mycotoxin production in a medium\u2010dependent manner. <jats:italic>rtfA<\/jats:italic> also affects the production of other metabolites including penicillin. Biosynthesis of this antibiotic decreased in the absence of <jats:italic>rtfA<\/jats:italic>. Furthermore, <jats:italic>rtfA<\/jats:italic> is necessary for normal morphological development. Deletion of the <jats:italic>rtfA<\/jats:italic> gene in wild\u2010type strains (<jats:italic>veA<\/jats:italic>+) resulted in a slight decrease in growth rate, drastic reduction in conidiation, and complete loss of sexual development. This is the first study of an Rtf1 like gene in filamentous fungi. We found <jats:italic>rtfA<\/jats:italic> putative orthologues extensively conserved in numerous fungal species.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.08142.x","type":"journal-article","created":{"date-parts":[[2012,7,12]],"date-time":"2012-07-12T11:27:06Z","timestamp":1342092426000},"page":"795-814","source":"Crossref","is-referenced-by-count":24,"title":["<i>veA<\/i>\u2010dependent RNA\u2010pol II transcription elongation factor\u2010like protein, RtfA, is associated with secondary metabolism and morphological development in <i>Aspergillus nidulans<\/i>"],"prefix":"10.1111","volume":"85","author":[{"given":"Vellaisamy","family":"Ramamoorthy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourabha","family":"Shantappa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourabh","family":"Dhingra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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