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In this study, the role of P66 in mammalian and tick infection was examined. <jats:italic>B.\u2003burgdorferi<\/jats:italic>\u0394<jats:italic>p66<\/jats:italic> strains were not infectious in wild\u2010type, TLR2<jats:sup>\u2212\/\u2212<\/jats:sup>\u2010 or MyD88<jats:sup>\u2212\/\u2212<\/jats:sup>\u2010deficient mice. Strains with <jats:italic>p66<\/jats:italic> restored to the chromosome restored near wild\u2010type infectivity, while complementation with <jats:italic>p66<\/jats:italic> on a shuttle vector did not restore infectivity. \u0394<jats:italic>p66<\/jats:italic> mutants are cleared quickly from the site of inoculation, but analyses of cytokine expression and cellular infiltrates at the site of inoculation did not reveal a specific mechanism of clearance. The defect in these mutants cannot be attributed to nutrient limitation or an inability to adapt to the host environment <jats:italic>in vivo<\/jats:italic> as \u0394<jats:italic>p66<\/jats:italic> bacteria were able to survive as well as wild type in dialysis membrane chambers in the rat peritoneum. \u0394<jats:italic>p66<\/jats:italic> bacteria were able to survive in ticks through the larva to nymph moult, but were non\u2010infectious in mice when delivered by tick bite. Independent lines of evidence do not support any increased susceptibility of the \u0394<jats:italic>p66<\/jats:italic> strains to factors in mammalian blood. This study is the first to define a <jats:italic>B.\u2003burgdorferi<\/jats:italic> adhesin as essential for mammalian, but not tick infection.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.08160.x","type":"journal-article","created":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T17:51:07Z","timestamp":1341337867000},"page":"1105-1118","source":"Crossref","is-referenced-by-count":52,"title":["The \u03b2<sub>3<\/sub>\u2010integrin ligand of <i>Borrelia burgdorferi<\/i> is critical for infection of mice but not ticks"],"prefix":"10.1111","volume":"85","author":[{"given":"Laura C.","family":"Ristow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halli E.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavinia 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