{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:44:22Z","timestamp":1791042262851,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,8,13]],"date-time":"2012-08-13T00:00:00Z","timestamp":1344816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Type IV pili are surface organelles essential for pathogenicity of many Gram\u2010negative bacteria. In <jats:italic>Neisseria gonorrhoeae<\/jats:italic>, the major subunit of type IV pili, PilE, is a target of its general <jats:italic>O<\/jats:italic>\u2010linked glycosylation system. This system modifies a diverse set of periplasmic and extracellular gonococcal proteins with a variable set of glycans. Here we show that expression of a particular hexa\u2010histidine\u2010tagged PilE was associated with growth arrest. By studying intra\u2010 and extragenic suppressors, we found that this phenotype was dependent on pilus assembly and retraction. Based on these results, we developed a sensitive tool to identify factors with subtle effects on pilus dynamics. Using this approach, we found that glycan chain length has differential effects on the growth arrest that appears to be mediated at the level of pilin subunit\u2013subunit interactions and bidirectional remodelling of pilin between its membrane\u2010associated and assembled states. Gonococcal pilin glycosylation thus plays both an intracellular role in pilus dynamics and potential extracellular roles mediated through type IV pili. In addition to demonstrating the effect of glycosylation on pilus dynamics, the study provides a new way of identifying factors with less dramatic effects on processes involved in type IV pilus biogenesis.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2012.08166.x","type":"journal-article","created":{"date-parts":[[2012,8,13]],"date-time":"2012-08-13T04:31:14Z","timestamp":1344832274000},"page":"1166-1178","source":"Crossref","is-referenced-by-count":20,"title":["Insights into type IV pilus biogenesis and dynamics from genetic analysis of a C\u2010terminally tagged pilin: a role for <i>O<\/i>\u2010linked glycosylation"],"prefix":"10.1111","volume":"85","author":[{"given":"\u00c5shild","family":"Vik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Aspholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan 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