{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:16:37Z","timestamp":1760548597208},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T00:00:00Z","timestamp":1201737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:p><jats:bold>Abstract\u2002<\/jats:bold>This study introduces a novel, simplified method for the evaluation of murine intestinal transit and contractility using fluorescence and video imaging. Intestinal transit was measured by evaluating the intestinal distribution of non\u2010absorbable fluorescein\u2010labelled dextran (70\u2003kDa, FD70) along the gastrointestinal (GI) tract. After excision of the GI tract, two full\u2010field images \u2013 one in normal illumination mode and another in fluorescent mode \u2013 were taken with a charge coupled device (CCD) camera and subsequently matched for calculation of fluorescence distribution along the GI tract. Immediately after, intestinal contractility was evaluated in different regions of the intact intestine by spatiotemporal motility mapping (i.e. video imaging). In control mice, the small intestine showed vigorous oscillatory contractions and FD70 was primarily distributed within the terminal ileum\/caecum at 90\u2003min postgavage. As validation step, the effect of intestinal manipulation (IM, surgical procedure) and two pharmacological agents \u2013 known to alter GI motility \u2013 was tested. At 24\u2003h postoperatively, spontaneous contractile activity of the small intestine was nearly abolished in IM mice, leaving the small intestine distended and resulting in a significantly delayed intestinal transit. In accordance, spontaneous mechanical activity of circular muscle strips in standard organ baths was significantly reduced in IM mice compared to control mice. Administration of atropine (1\u20133\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, i.p.) suppressed spontaneous contractile activity along the entire intestinal tract and induced a dose\u2010related delay in intestinal transit. In contrast, metoclopramide (3\u201310\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, i.p.) markedly increased contractile activity \u2013 however only in the upper GI tract \u2013 and accelerated intestinal transit in a dose\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2007.01073.x","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T18:25:46Z","timestamp":1201803946000},"page":"700-707","source":"Crossref","is-referenced-by-count":18,"title":["A novel method for the evaluation of intestinal transit and contractility in mice using fluorescence imaging and spatiotemporal motility mapping"],"prefix":"10.1111","volume":"20","author":[{"given":"O.","family":"De Backer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Blanckaert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Leybaert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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