{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:02:27Z","timestamp":1788382947394,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p><jats:bold>Abstract\u2002<\/jats:bold> The afferent neurons innervating the oesophagus originate from two embryonic sources: neurons located in vagal nodose ganglia originate from embryonic placodes and neurons located in vagal jugular and spinal dorsal root ganglia (DRG) originate from the neural crest. Here, we address the hypothesis that 5\u2010hydroxytryptamine (5\u2010HT) differentially stimulates afferent nerve subtypes in the oesophagus. Extracellular recordings of single unit activity originating from nerve terminals were made in the isolated innervated guinea\u2010pig oesophagus. Whole cell patch clamp recordings (35\u2003\u00b0C) were made from the primary afferent neurons retrogradely labelled from the oesophagus. 5\u2010Hydroxytryptamine (10\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup>) activated vagal nodose C\u2010fibres (70%) in the oesophagus but failed to activate overtly vagal jugular nerve fibres and oesophagus\u2010specific spinal DRG neurons. The response to 5\u2010HT in nodose C\u2010fibre nerve terminals was mimicked by the selective 5\u2010HT<jats:sub>3<\/jats:sub> receptor agonist 2\u2010methyl\u20105\u2010HT (10\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup>) and nearly abolished by the 5\u2010HT<jats:sub>3<\/jats:sub> receptor antagonists ondansetron (10\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup>) and Y\u201025130 (10\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup>). In patch clamp studies, 2\u2010methyl\u20105\u2010HT (10\u2003<jats:italic>\u03bc<\/jats:italic>mol\u2003L<jats:sup>\u22121<\/jats:sup>) activated a proportion of isolated oesophagus\u2010specific nodose capsaicin\u2010sensitive neurons (putative cell bodies of nodose C\u2010fibres). We conclude that the responsiveness to 5\u2010HT discriminates placode\u2010derived (vagal nodose) C\u2010fibres from the neural crest\u2010derived (vagal jugular and spinal DRG) afferent nerves in the oesophagus. The response to 5\u2010HT in nodose C\u2010fibres is mediated by the 5\u2010HT<jats:sub>3<\/jats:sub> receptor in their neuronal membrane.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2008.01136.x","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T07:22:46Z","timestamp":1210836166000},"page":"1042-1050","source":"Crossref","is-referenced-by-count":28,"title":["5\u2010Hydroxytryptamine selectively activates the vagal nodose C\u2010fibre subtype in the guinea\u2010pig oesophagus"],"prefix":"10.1111","volume":"20","author":[{"given":"S.","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kollarik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,13]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.11.002"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.51.suppl_1.i81"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01076212"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.907bd.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00785.2001"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(93)90361-W"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(93)90360-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02300369"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1989.61.5.1001"},{"key":"e_1_2_7_11_2","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1152\/jn.1992.68.4.1053","article-title":"Differential sensitivity to bradykinin of esophageal distension\u2010sensitive mechanoreceptors in vagal and sympathetic afferents of the opossum","volume":"68","author":"Sengupta JN","year":"1992","journal-title":"J Neurophysiol"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1990.64.3.796"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00277.2007"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.079574"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0156"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1192"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.109371"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-16-06249.2000"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.051862"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1984.sp015022"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019889"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00206.2001"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.130823"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1201\/9780203492314.pt6"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(00)00314-0"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.07.016"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2982.2008.01136.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2982.2008.01136.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T06:42:37Z","timestamp":1697265757000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2982.2008.01136.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,13]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1111\/j.1365-2982.2008.01136.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2982.2008.01136.x","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,13]]}}}