{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:28:11Z","timestamp":1791156491410,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,9]],"date-time":"2011-11-09T00:00:00Z","timestamp":1320796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa\u2010associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa\u2010associated microbiota between patients with diarrhea predominant IBS (IBS\u2010D), constipation predominant IBS (IBS\u2010C) and controls using fluorescent <jats:italic>in situ<\/jats:italic> hybridization and to correlate specific bacteria groups with individual IBS symptoms.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Forty\u2010seven patients with IBS (27 IBS\u2010D and 20 IBS\u2010C) and 26 healthy controls were recruited to the study. Snap\u2010frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial\u2010group specific oligonucleotide probes.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> Patients with IBS had significantly greater numbers of total mucosa\u2010associated bacteria per mm of rectal epithelium than controls [median 218 (IQR \u2013 209) <jats:italic>vs<\/jats:italic> 128 (121) <jats:italic>P<\/jats:italic>\u2003=\u20030.007], and this was chiefly comprised of bacteroides IBS [69 (67) <jats:italic>vs<\/jats:italic> 14 (41) <jats:italic>P<\/jats:italic>\u2003=\u20030.001] and <jats:italic>Eubacterium rectale<\/jats:italic>\u2013<jats:italic>Clostridium coccoides<\/jats:italic> [52 (58) <jats:italic>vs<\/jats:italic> 25 (35) <jats:italic>P<\/jats:italic>\u2003=\u20030.03]. Analysis of IBS sub\u2010groups demonstrated that bifidobacteria were lower in the IBS\u2010D group than in the IBS\u2010C group and controls [24 (32) <jats:italic>vs<\/jats:italic> 54 (88) <jats:italic>vs<\/jats:italic> 32 (35) <jats:italic>P<\/jats:italic>\u2003=\u20030.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa\u2010associated bifidobacteria (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) and lactobacilli (<jats:italic>P<\/jats:italic>\u2003=\u20030.002).<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> The mucosa\u2010associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS\u2010D.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2011.01803.x","type":"journal-article","created":{"date-parts":[[2011,11,10]],"date-time":"2011-11-10T01:30:59Z","timestamp":1320888659000},"page":"31-39","source":"Crossref","is-referenced-by-count":179,"title":["Distinct microbial populations exist in the mucosa\u2010associated microbiota of sub\u2010groups of irritable bowel syndrome"],"prefix":"10.1111","volume":"24","author":[{"given":"G. C.","family":"Parkes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. B.","family":"Rayment","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Hudspith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Petrovska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Lomer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Brostoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Whelan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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