{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T07:37:48Z","timestamp":1724312268933},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,2,3]],"date-time":"2012-02-03T00:00:00Z","timestamp":1328227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Colonic dysfunction occurs after pelvic autonomic nerve damage. The enteric nervous system can compensate. We investigated the role of mucosal serotonin receptors, 5\u2010HT<jats:sub>3<\/jats:sub> and 5\u2010HT<jats:sub>4<\/jats:sub>, in the colonic motility restoration over 2 weeks after parasympathetic pelvic nerve transection in a rat model.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Male Sprague\u2010Dawley rats underwent pelvic nerve transection or sham operation. Colonic transit was expressed as the geometric center of <jats:sup>51<\/jats:sup>Cr distribution. Mucosal 5\u2010HT<jats:sub>3<\/jats:sub> and 5\u2010HT<jats:sub>4<\/jats:sub> receptor expression was evaluated by Western blot. Intraluminal pressure increase was measured after 5\u2010HT<jats:sub>3<\/jats:sub> (ondansetron) or 5\u2010HT<jats:sub>4<\/jats:sub> receptor antagonist (GR125487) administration <jats:italic>in vitro<\/jats:italic> in sham and denervated distal colons.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> At 2 weeks, colonic transit in the denervated group was 30% slower compared to the sham group (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.01). At 1 and 2 weeks, 5\u2010HT<jats:sub>3<\/jats:sub> receptor expression was increased two\u2010fold in the denervated group, compared to shams (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.05). A three\u2010fold smaller dose of ondansetron was required in denervated tissues to inhibit intraluminal pressure rise than in sham colons (<jats:italic>P\u2003<\/jats:italic>&lt;\u20030.01). There was no difference in the expression of 5\u2010HT<jats:sub>4<\/jats:sub> receptor or the response to GR125487 in denervated <jats:italic>vs<\/jats:italic> sham colons.<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> Colonic motility was restored to approximately 70% normal over 1 week without further improvement at 2 weeks. Enteric nervous system compensated by upregulating mucosal 5\u2010HT<jats:sub>3,<\/jats:sub> but not 5\u2010HT<jats:sub>4,<\/jats:sub> receptors.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2012.01890.x","type":"journal-article","created":{"date-parts":[[2012,2,3]],"date-time":"2012-02-03T12:22:48Z","timestamp":1328271768000},"page":"472-478","source":"Crossref","is-referenced-by-count":4,"title":["Upregulation of mucosal 5\u2010HT<sub>3<\/sub> receptors is involved in restoration of colonic transit after pelvic nerve transection"],"prefix":"10.1111","volume":"24","author":[{"given":"I.","family":"Gribovskaja\u2010Rupp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Ridolfi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Kosinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ludwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,3]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02554543"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1987.67.3.902"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01400.x"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(74)80114-9"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2004.03.022"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005665218647"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2001.01699.x"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10350-004-0814-6"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2008.03.014"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2006.00347.x"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1993.1311"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2011.560682"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2010.05.002"},{"key":"e_1_2_9_15_2","first-page":"491","article-title":"Innervation of the pelvic organs of the rat","volume":"2","author":"Langworthy OR","year":"1965","journal-title":"Invest Urol"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00419.2006"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(01)00317-4"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.2000.00198.x"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018827613809"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-009-0999-z"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2168.1997.00578.x"},{"key":"e_1_2_9_23_2","first-page":"854","article-title":"Colonic J\u2010pouch decreases bowel frequency by improving the evacuation ratio","volume":"53","author":"Kimura H","year":"2006","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-011-1489-x"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01461.x"},{"issue":"3","key":"e_1_2_9_26_2","first-page":"613","article-title":"An ultrastructural study of the submucous plexus of guinea pig intestine after unilateral vagotomy","volume":"187","author":"Wang XY","year":"1995","journal-title":"J Anat"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4695"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.025452"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcg.0000156403.37240.30"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10350-006-0763-3"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.11.002"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70203-3"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.02180.x"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34245"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00775-3"}],"container-title":["Neurogastroenterology &amp; 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