{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T05:10:38Z","timestamp":1794373838248,"version":"4.9.0"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,20]],"date-time":"2012-02-20T00:00:00Z","timestamp":1329696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> The intestinal microbiota has been implicated in the pathophysiology of irritable bowel syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are inconsistent. We analyzed the composition of the intestinal microbiota in a defined subgroup of IBS patients (diarrhea\u2010predominant IBS, D\u2010IBS) using a technique that provides the deepest characterization available for complex microbial communities.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Fecal DNA was isolated from 23 D\u2010IBS patients and 23 healthy controls (HC). Variable regions V1\u2013V3 and V6 of the 16S rRNA gene were amplified from all samples. PCR products were sequenced using 454 high throughput sequencing. The composition, diversity and richness of microbial communities were determined and compared between D\u2010IBS and HC using the quantitative insights into microbial ecology pipeline.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> The contribution of bacterial groups to the composition of the intestinal microbiota differed between D\u2010IBS and HC. D\u2010IBS patients had significantly higher levels of <jats:italic>Enterobacteriaceae<\/jats:italic> (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.03), and lower levels of <jats:italic>Fecalibacterium<\/jats:italic> genera (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.04) compared to HC. \u03b2\u2010Diversity values demonstrated significantly lower levels of UniFrac distances in HC compared to D\u2010IBS patients. The richness of 16S rRNA sequences was significantly decreased in D\u2010IBS patients (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.04).<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> Our 16S rRNA sequence data demonstrates a community\u2010level dysbiosis in D\u2010IBS. The altered composition of the intestinal microbiota in D\u2010IBS is associated with significant increases in detrimental and decreases in beneficial bacterial groups, and a reduction in microbial richness.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2012.01891.x","type":"journal-article","created":{"date-parts":[[2012,2,20]],"date-time":"2012-02-20T07:45:44Z","timestamp":1329723944000},"page":"521","source":"Crossref","is-referenced-by-count":380,"title":["Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea\u2010predominant irritable bowel syndrome"],"prefix":"10.1111","volume":"24","author":[{"given":"I. 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