{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T12:07:06Z","timestamp":1793362026792,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T00:00:00Z","timestamp":1329868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Irritable bowel syndrome (IBS) is a multifactorial disease for which a dysbiosis of the gut microbiota has been described. Bile acids (BA) could play a role as they are endogenous laxatives and are metabolized by gut microbiota. We compared fecal BA profiles and microbiota in healthy subjects (HS) and patients with diarrhea\u2010predominant IBS (IBS\u2010D), and we searched for an association with symptoms.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Clinical features and stool samples were collected in IBS\u2010D patients and HS. Fecal BA profiles were generated using HPLC coupled to tandem mass spectrometry. The fecal microbiota composition was assessed by q\u2010PCR targeting dominant bacterial groups and species implicated in BA transformation.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> Fourteen IBS\u2010D patients and 18 HS were included. The two groups were comparable in terms of age and sex. The percentage of fecal primary BA was significantly higher in IBS\u2010D patients than in HS, and it was significantly correlated with stool consistency and frequency. Fecal counts of all bacteria, <jats:italic>lactobacillus, coccoides, leptum<\/jats:italic> and <jats:italic>Faecalibacterium prausnitzii<\/jats:italic> were similar. There was a significant increase of <jats:italic>Escherichia coli<\/jats:italic> and a significant decrease of <jats:italic>leptum<\/jats:italic> and <jats:italic>bifidobacterium<\/jats:italic> in IBS\u2010D patients.<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> We report an increase of primary BA in the feces of IBS\u2010D patients compared to HS, correlated with stool consistency and frequency. A dysbiosis of different bacterial groups was detected, some of them involved in BA transformation. As the gut microbiota is the exclusive pathway to transform primary into secondary BA, this suggests a functional consequence of dysbiosis, leading to lower BA transformation.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2012.01893.x","type":"journal-article","created":{"date-parts":[[2012,2,23]],"date-time":"2012-02-23T01:18:26Z","timestamp":1329959906000},"page":"513","source":"Crossref","is-referenced-by-count":229,"title":["Increase in fecal primary bile acids and dysbiosis in patients with diarrhea\u2010predominant irritable bowel syndrome"],"prefix":"10.1111","volume":"24","author":[{"given":"H.","family":"Duboc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Rainteau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Rajca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Humbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Farabos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Maubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Grondin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Jouet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Bouhassira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Seksik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Sokol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Coffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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