{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:13:34Z","timestamp":1785773614213,"version":"3.56.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T00:00:00Z","timestamp":1334016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> In obstructive bowel disorders (OBDs) such as achalasia, pyloric stenosis, and bowel obstruction, the lumen of the affected segments is markedly dilated and the motility function is significantly impaired. We tested the hypothesis that mechanical stress in lumen dilation leads to induction of cyclooxygenase\u20102 (COX\u20102) in smooth muscle throughout the gastrointestinal (GI) tract, contributing to motility dysfunction.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Lumen dilation was induced <jats:italic>in vivo<\/jats:italic> with obstruction bands (12\u2003\u00d7\u20033\u2003mm) applied over the lower esophageal sphincter (LES), the pyloric sphincter, and the ileum in rats for 48\u2003h. Mechanical stretch <jats:italic>in vivo<\/jats:italic> was also emulated by balloon distension of the distal colon. Direct stretch of muscle strips from the esophagus, gastric fundus, and ileum was mimicked in an <jats:italic>in vitro<\/jats:italic> tissue culture system.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> Partial obstruction in the LES, pylorus, and ileum significantly increased the expression of COX\u20102 mRNA and protein in the muscularis externae of the dilated segment oral to the occlusions, but not in the aboral segment. Direct stretch of the lumen <jats:italic>in vivo<\/jats:italic> or of muscle strips <jats:italic>in vitro<\/jats:italic> markedly induced COX\u20102 expression. The smooth muscle contractility was significantly suppressed in the balloon\u2010distended segments. However, treatment with COX\u20102 inhibitor NS\u2010398 restored the contractility. Furthermore<jats:italic>, in vivo<\/jats:italic> administration of NS\u2010398 in gastric outlet obstruction significantly improved gastric emptying.<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> Mechanical dilation of the gut lumen by occlusion or direct distension induces gene expression of COX\u20102 throughout the GI tract. Mechanical stress\u2010induced COX\u20102 contributes to motility dysfunction in conditions with lumen dilation.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2012.01918.x","type":"journal-article","created":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T03:59:25Z","timestamp":1334116765000},"page":"670","source":"Crossref","is-referenced-by-count":19,"title":["Mechano\u2010transcription of COX\u20102 is a common response to lumen dilation of the rat gastrointestinal 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