{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:53:40Z","timestamp":1790787220359,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T00:00:00Z","timestamp":1337817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Congenital aganglionosis (Hirschsprung\u2019s disease) results in colonic dysmotility and a risk for Hirschsprung\u2019s\u2010associated enterocolitis (HAEC), whose cause is unknown. We hypothesized that aganglionosis leads to microbiome changes that may contribute to HAEC risk.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Colon and fecal samples were collected from endothelin receptor B\u2010null (Ednrb<jats:sup>\u2212\/\u2212<\/jats:sup>) mice, an established model of colorectal aganglionosis, at postnatal day 7 (P7), P20, and P24. We determined microbiome composition by 16S ribosomal RNA gene pyrosequencing and fecal metabolite profile by nuclear magnetic resonance spectroscopy.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> Wild\u2010type (WT) mice exhibited increasing species diversity with age, with mutant mice possessing even greater diversity. WT and mutant microbiomes, both fecal and colonic, significantly segregated by principal coordinates analysis based on species composition at all ages examined. Importantly, mutant mice contained more Bacteroidetes and less Firmicutes than WT, with additional genus\u2010 and species\u2010level differences observed. Notably, mutant P7 colon was dominated by coagulase\u2010negative <jats:italic>Staphylococcus<\/jats:italic> species, which were rare in WT. Mutant fecal metabolite profiles also differed, particularly in the abundance of formate, a short\u2010chain fatty acid product of microbial fermentation.<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> Colorectal aganglionosis is associated with early and sustained disruption of the normal colonic and fecal microbiome, supporting the enteric nervous system as a determinant of microbiome composition. Furthermore, the differences observed suggest a potential contributory role for the microbiome in the etiology of HAEC. These findings provide a basis for further studies to determine the causative role of specific bacterial communities in HAEC and the potential to restore the normal microbiome in Hirschsprung\u2019s disease.<\/jats:p>","DOI":"10.1111\/j.1365-2982.2012.01937.x","type":"journal-article","created":{"date-parts":[[2012,5,25]],"date-time":"2012-05-25T03:15:27Z","timestamp":1337915727000},"page":"874","source":"Crossref","is-referenced-by-count":71,"title":["Intestinal aganglionosis is associated with early and sustained disruption of the colonic microbiome"],"prefix":"10.1111","volume":"24","author":[{"given":"N. L.","family":"Ward","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Pieretti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. E.","family":"Dowd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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