{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:09:39Z","timestamp":1791223779463,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,6]],"date-time":"2005-10-06T00:00:00Z","timestamp":1128556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p>Nerve cells are highly susceptible to ischemic and hypoxic injuries. The neuroglobin (Ngb), found in vertebrate nerve cells, has been suggested to protect nerve cells from ischemic episodes by a yet unknown mechanism. However, contradicting reports exist regarding localization and up\u2010regulation of Ngb in response to hypoxia. The aim of the present study was to probe the distribution of Ngb proteins in mouse brain and retina by immunohistochemistry, and to quantify the levels of Ngb mRNA by reverse\u2010transcription\u2010polymerase chain reaction (RT\u2010PCR) after short\u2010term (2\u2003h) exposure to 7.6% oxygen. We found Ngb to be present throughout the neocortex, most abundantly in the perirhinal, entorhinal and temporal cortical areas, the thalamus and hypothalamus, the choroid plexus, the olfactory bulb and the cranial nerve nuclei in the brainstem. Intense staining was observed in the mesencephalic central grey area and the Purkinje cells. Two\u2010hour hypoxic exposure caused no detectable changes in staining intensity or spatial distribution of Ngb neither in the Purkinje cells nor in any other brain areas observed. The RT\u2010PCR data supported the lack of differences in brain Ngb levels between normal and oxygen\u2010deprived animals. In the retina, Ngb localization by immunohistochemistry was confined to the inner segments of the photoreceptors, the plexiform layers and the ganglion cells. Short\u2010termed hypoxia did not change retinal Ngb levels as assessed by both techniques. The lack of Ngb up\u2010regulation in the brain is consistent with results from previous long\u2010term hypoxic experiments, suggesting that Ngb is not regulated by pure hypoxia <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2005.00657.x","type":"journal-article","created":{"date-parts":[[2005,10,6]],"date-time":"2005-10-06T08:12:17Z","timestamp":1128586337000},"page":"610-617","source":"Crossref","is-referenced-by-count":48,"title":["Effects of short\u2010term hypoxia on neuroglobin levels and localization in mouse brain tissues"],"prefix":"10.1111","volume":"31","author":[{"given":"C.","family":"Hundahl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Stoltenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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Aldolase C, EAAT4, and the cerebellar contribution to posthypoxic myoclonus","volume":"89","author":"Welsh JP","year":"2002","journal-title":"Adv Neurol"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00253-2"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C300203200"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf248"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309396200"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2307\/1541760"},{"key":"e_1_2_6_25_2","first-page":"149","article-title":"Selective vulnerability in brain hypoxia","volume":"6","author":"Cervosnavarro J","year":"1991","journal-title":"Crit Rev Neurobiol"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02613-0"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313732200"},{"key":"e_1_2_6_28_2","doi-asserted-by":"crossref","unstructured":"FagoA HundahlC DewildeS GilanyK MoensL WeberRE.Allosteric regulation and temperature dependence of oxygen binding in human neuroglobin and cytoglobin. 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