{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:15:58Z","timestamp":1790856958800,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,10,6]],"date-time":"2005-10-06T00:00:00Z","timestamp":1128556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p>Multidrug transporters, such as P\u2010glycoprotein (P\u2010gp), multidrug\u2010resistance associated protein 1 (MRP1) and breast cancer resistance protein (BCRP), are associated with multidrug resistance in cancers; other molecules, such as major vault protein (MVP), have a similar association with drug\u2010resistant cancer. These proteins are postulated to generate drug resistance in epilepsy. They have been shown individually to be up\u2010regulated in epileptogenic brain tissue. In any consideration of the function, inhibition or evasion of the activity of such proteins, the colocalization of such proteins needs to be understood. We systematically determined the presence of such colocalization, focusing on microvascular endothelium from epileptogenic human brain tissue. Double labelling immunofluorescence and confocal laser scanning microscopy were used to determine colocalization of P\u2010gp, MRP1, BCRP and MVP in one case of hippocampal sclerosis and two cases of focal cortical dysplasia type IIb. Endothelial colocalization was examined with double labelling using antibodies to CD34 and Factor VIII. The presence of P\u2010gp, BCRP and MVP in microvascular endothelium was confirmed. P\u2010gp, BCRP and MVP colocalized in microvascular endothelium, though not all proteins appeared to be identically distributed within this tissue. MRP1 did not colocalize to endothelium. These findings were not unexpected but required formal confirmation. The demonstrated colocalization of P\u2010gp, BCRP and MVP in microvascular endothelium in epileptogenic human brain tissue has important implications for functional experiments (including single knock\u2010out mice studies), work with specific and broad\u2010spectrum inhibitors of transport function, and any eventual trials of treatment of refractory epilepsy involving modulation of the function of these proteins.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2005.00699.x","type":"journal-article","created":{"date-parts":[[2005,10,6]],"date-time":"2005-10-06T08:12:17Z","timestamp":1128586337000},"page":"51-63","source":"Crossref","is-referenced-by-count":73,"title":["Vascular colocalization of P\u2010glycoprotein, multidrug\u2010resistance associated protein 1, breast cancer resistance protein and major vault protein in human epileptogenic pathologies"],"prefix":"10.1111","volume":"32","author":[{"given":"S. M.","family":"Sisodiya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Martinian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. L.","family":"Scheffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Van Der Valk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Scheper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Harding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Thom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10473"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-200304000-00013"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013-9580.2005.31904.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(98)00061-8"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00212-3"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/0470846356.ch3"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118699"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3900"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200211150-00014"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1528-1157.2003.21803.x"},{"key":"e_1_2_6_12_2","first-page":"3458","article-title":"Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues","volume":"61","author":"Maliepaard M","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0695-578"},{"key":"e_1_2_6_14_2","first-page":"1475","article-title":"Overexpression of a M(r) 110,000 vesicular protein in non\u2010P\u2010glycoprotein\u2010mediated multidrug resistance","volume":"53","author":"Scheper RJ","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-200011000-00007"},{"key":"e_1_2_6_16_2","first-page":"877","article-title":"Broad distribution of the multidrug resistance\u2010related vault lung resistance protein in normal human tissues and tumors","volume":"148","author":"Izquierdo MA","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi047948g"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1995.tb01657.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199911080-00032"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awf002"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00992-2"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013-9580.2004.57703.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.301.1.7"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-14-05833.2002"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-2-37"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03573-X"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2005.66604.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000114507.30388.7E"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1E068"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1528-1157.2002.156702.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403395"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000901)87:5<615::AID-IJC1>3.0.CO;2-4"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200108080-00020"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/0470846356.ch4"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.32.7.722"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000026420.79421.8f"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0914-3"}],"container-title":["Neuropathology and Applied Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2990.2005.00699.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2990.2005.00699.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:13:50Z","timestamp":1696983230000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2990.2005.00699.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,4]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1111\/j.1365-2990.2005.00699.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2990.2005.00699.x","archive":["Portico"],"relation":{},"ISSN":["0305-1846","1365-2990"],"issn-type":[{"value":"0305-1846","type":"print"},{"value":"1365-2990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,4]]}}}