{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:14:39Z","timestamp":1767831279408,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,12]],"date-time":"2007-01-12T00:00:00Z","timestamp":1168560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:p>The four GPI\u2010anchored cell adhesion molecules that exemplify the IgLON family are most highly expressed in the nervous system and associate to form up to six different heterodimeric \u2018Diglons\u2019 that can modify cell adhesion and inhibit axon migration. Recently, two members, <jats:italic>OPCML<\/jats:italic> and <jats:italic>LSAMP<\/jats:italic>, were identified as putative tumour suppressor genes in ovarian and renal carcinomas respectively. In this study, we investigated <jats:italic>OPCML<\/jats:italic> expression in nonneoplastic brain tissue and 35 brain tumours (18 glioblastoma multiformes, five anaplastic gliomas, five meningiomas, six metastases and one medulloblastoma) and four glioma cell lines using quantitative reverse transcriptase polymerase chain reaction (RT\u2010PCR). <jats:italic>OPCML<\/jats:italic> was highly expressed in cerebellum, less so in cerebral cortex, frontal lobe and meninges and was significantly reduced or absent in 83% of brain tumours and all cell lines compared with nonneoplastic whole brain. Two <jats:italic>OPCML<\/jats:italic> splice variants have been identified in humans, termed \u03b11 and \u03b12, but the latter has not been demonstrated in human neural tissues. Using PCR with specific primers, nonneoplastic brain and 3\/6 of tested brain tumours expressed both splice variants, whereas the remaining brain tumours only expressed the \u03b12 variant. Hypermethylation of the \u03b11 <jats:italic>OPCML<\/jats:italic> promoter, associated with down\u2010regulation of expression in ovarian tumours, did not correlate with expression levels in the subset of brain tumours tested, implying transcription of <jats:italic>OPCML<\/jats:italic> from an alternative promoter or a different mechanism of down\u2010regulation. This study demonstrates that <jats:italic>OPCML<\/jats:italic> down\u2010regulation occurs in the majority of brain tumours tested, warranting further investigation of <jats:italic>OPCML<\/jats:italic> and other IgLONs in the development and progression of brain tumours.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2006.00786.x","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T18:23:47Z","timestamp":1168712627000},"page":"77-85","source":"Crossref","is-referenced-by-count":44,"title":["Expression of cellular adhesion molecule \u2018<i>OPCML<\/i>\u2019 is down\u2010regulated in gliomas and other brain tumours"],"prefix":"10.1111","volume":"33","author":[{"given":"J. E.","family":"Reed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Du Plessis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. J.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Reeves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. L.","family":"Gee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. C.","family":"Warnke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. C.","family":"Sellar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Moss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00237-9"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.12.8224"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-22-09312.1998"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-3042.2002.00798.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1242\/jcs.109.13.3129","article-title":"A family of glycoproteins (GP55), which inhibit neurite outgrowth, are members of the Ig superfamily and are related to OBCAM, neurotrimin, LAMP and CEPU\u20101","volume":"109","author":"Wilson DJ","year":"1996","journal-title":"J Cell Sci"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.04.010"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/neu.10050"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(00)00184-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.4.865"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1242\/dev.121.4.1161","article-title":"The limbic system\u2010associated membrane protein (LAMP) selectively mediates interactions with specific central neuron populations","volume":"121","author":"Zhukareva V","year":"1995","journal-title":"Development"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01261"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1183"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4586fje"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2388"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00269-1"},{"key":"e_1_2_6_17_2","volume-title":"Pathology and Genetics of Tumors of the Nervous System","author":"Kleihues P","year":"2000"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12328-8"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.04.5302"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.05.063"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1215\/15228517-4-3-179"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.089"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603006"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(00)00072-9"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhi109"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00873-4"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.01.013"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-004-2751-6"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024520"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(00)80122-3"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050696"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(94)00178-Q"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980518)76:4<451::AID-IJC1>3.0.CO;2-Q"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19980515)82:10<1921::AID-CNCR16>3.0.CO;2-V"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1098"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1593\/neo.04535"}],"container-title":["Neuropathology and Applied Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2990.2006.00786.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2990.2006.00786.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T12:13:47Z","timestamp":1697026427000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2990.2006.00786.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,12]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1111\/j.1365-2990.2006.00786.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2990.2006.00786.x","archive":["Portico"],"relation":{},"ISSN":["0305-1846","1365-2990"],"issn-type":[{"value":"0305-1846","type":"print"},{"value":"1365-2990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,12]]}}}