{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T01:07:25Z","timestamp":1789780045704,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,30]],"date-time":"2008-12-30T00:00:00Z","timestamp":1230595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p> <jats:bold>Aims:<\/jats:bold> Increased endothelial caveolae leading to transcytosis of plasma proteins is associated with blood\u2013brain barrier (BBB) breakdown and cerebral oedema in brain injury. Increased expression of caveolin\u20101\u03b1 (Cav\u20101), an integral caveolar membrane protein, was reported in endothelium of arterioles and veins with BBB breakdown to fibronectin post injury. In this study the phosphorylation state of Cav\u20101 and its association with BBB breakdown was determined in the rat cortical cold injury model over a period of days 0.5\u20136 post lesion. <jats:bold>Methods:<\/jats:bold> Expression of phosphorylated Cav\u20101 was determined by immunoblotting and dual labelling immunofluorescence for phosphorylated caveolin\u20101 and fibronectin, a marker of BBB breakdown. A phospho\u2010specific monoclonal antibody that selectively recognizes only tyrosine 14\u2010phosphorylated Cav\u20101 (PY14Cav\u20101) was used. <jats:bold>Results:<\/jats:bold> Immunoblots showed constitutive expression of PY14Cav\u20101 in cortex of control rats and a significant increase in PY14Cav\u20101 expression at the lesion site up to day 4 post lesion. PY14Cav\u20101 immunostaining was observed in the endothelium of lesion vessels at days 0.5\u20134 post lesion, in neutrophils at days 0.5 and 2 and in macrophages at day 6 post lesion. Dual labelling showed that 100% of vessels with BBB breakdown to fibronectin showed endothelial PY14Cav\u20101 on day 0.5, the percentage decreasing to 62% on day 4. On day 6, none of the vessels showed endothelial phosphorylated Cav\u20101. <jats:bold>Conclusions:<\/jats:bold> The presence of phosphorylated Cav\u20101 in endothelium of vessels showing BBB breakdown suggests that phosphorylated Cav\u20101 signalling may be one of the factors associated with early BBB breakdown and brain oedema in brain injury.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2008.01009.x","type":"journal-article","created":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T15:48:05Z","timestamp":1230824885000},"page":"417-426","source":"Crossref","is-referenced-by-count":78,"title":["Expression of endothelial phosphorylated caveolin\u20101 is increased in brain injury"],"prefix":"10.1111","volume":"35","author":[{"given":"S.","family":"Nag","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Manias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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