{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:56:55Z","timestamp":1790312215706,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,9,17]],"date-time":"2012-09-17T00:00:00Z","timestamp":1347840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Y. Chiba, S. Takei, N. Kawamura, Y. Kawaguchi, K. Sasaki, S. Hasegawa\u2010Ishii, A. Furukawa, M. Hosokawa and A. Shimada (2012) <jats:italic>Neuropathology and Applied Neurobiology<\/jats:italic><jats:bold>38,<\/jats:bold> 559\u2013571<\/jats:p><jats:p><jats:bold>Immunohistochemical localization of aggresomal proteins in glial cytoplasmic inclusions in multiple system atrophy<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:<\/jats:bold> Multiple system atrophy (MSA) is pathologically characterized by the formation of \u03b1\u2010synuclein\u2010containing glial cytoplasmic inclusions (GCIs) in oligodendrocytes. However, the mechanisms of GCI formation are not fully understood. Cellular machinery for the formation of aggresomes has been linked to the biogenesis of the Lewy body, a characteristic \u03b1\u2010synuclein\u2010containing inclusion of Parkinson's disease and dementia with Lewy bodies. Here, we examined whether GCIs contain the components of aggresomes by immunohistochemistry. <jats:bold>Methods:<\/jats:bold> Sections from five patients with MSA were stained immunohistochemically with antibodies against aggresome\u2010related proteins and analysed in comparison with sections from five patients with no neurological disease. We evaluated the presence or absence of aggresome\u2010related proteins in GCIs by double immunofluorescence and immunoelectron microscopy. <jats:bold>Results:<\/jats:bold> GCIs were clearly immunolabelled with antibodies against aggresome\u2010related proteins, such as \u03b3\u2010tubulin, histone deacetylase 6 (HDAC6) and 20S proteasome subunits. Neuronal cytoplasmic inclusions (NCIs) were also immunopositive for these aggresome\u2010related proteins. Double immunofluorescence staining and quantitative analysis demonstrated that the majority of GCIs contained these proteins, as well as other aggresome\u2010related proteins, such as Hsp70, Hsp90 and 62\u2010kDa protein\/sequestosome 1 (p62\/SQSTM1). Immunoelectron microscopy demonstrated immunoreactivities for \u03b3\u2010tubulin and HDAC6 along the fibrils comprising GCIs. <jats:bold>Conclusions:<\/jats:bold> Our results indicate that GCIs, and probably NCIs, share at least some characteristics with aggresomes in terms of their protein components. Therefore, GCIs and NCIs may be another manifestation of aggresome\u2010related inclusion bodies observed in neurodegenerative diseases.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2011.01229.x","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T11:42:30Z","timestamp":1319197350000},"page":"559-571","source":"Crossref","is-referenced-by-count":43,"title":["Immunohistochemical localization of aggresomal proteins in glial cytoplasmic inclusions in multiple system 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