{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:04:41Z","timestamp":1781175881427,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>G. R. Campbell, A. Reeve, I. Ziabreva, T. M. Polvikoski, R. W. Taylor, R. Reynolds, D. M. Turnbull and D. J. Mahad (2013) <jats:italic>Neuropathology and Applied Neurobiology<\/jats:italic><jats:bold>39,<\/jats:bold> 377\u2013389<\/jats:p><jats:p><jats:bold>Mitochondrial DNA deletions and depletion within paraspinal muscles<\/jats:bold><\/jats:p><jats:p><jats:bold>Aims:<\/jats:bold> Although mitochondrial abnormalities have been reported within paraspinal muscles in patients with axial weakness and neuromuscular disease as well as with ageing, the basis of respiratory deficiency in paraspinal muscles is not known. This study aimed to determine the extent and basis of respiratory deficiency in paraspinal muscles from cases undergoing surgery for degenerative spinal disease and <jats:italic>post mortem<\/jats:italic> cases without a history of spinal disease, where age\u2010related histopathological changes were previously reported. <jats:bold>Methods:<\/jats:bold> Cervical and lumbar paraspinal muscles were obtained peri\u2010operatively from 13 patients and from six <jats:italic>post mortem<\/jats:italic> control cases (age range 18\u201382 years) without a neurological disease. Sequential COX\/SDH (mitochondrial respiratory chain complex IV\/complex II) histochemistry was performed to identify respiratory\u2010deficient muscle fibres (lacking complex IV with intact complex II activity). Real\u2010time polymerase chain reaction, long\u2010range polymerase chain reaction and sequencing were used to identify and characterize mitochondrial DNA (mtDNA) deletions and determine mtDNA copy number status. Mitochondrial respiratory chain complex subunits were detected by immunohistochemistry. <jats:bold>Results:<\/jats:bold> The density of respiratory\u2010deficient fibres increased with age. On average, 3.96% of fibres in paraspinal muscles were respiratory\u2010deficient (range 0\u201310.26). Respiratory deficiency in 36.8% of paraspinal muscle fibres was due to clonally expanded mtDNA deletions. MtDNA depletion accounted for further 13.5% of respiratory deficiency. The profile of immunohistochemically detected subunits of complexes was similar in respiratory\u2010deficient fibres with and without mtDNA deletions or mtDNA depletion. <jats:bold>Conclusions:<\/jats:bold> Paraspinal muscles appeared to be particularly susceptible to age\u2010related mitochondrial respiratory chain defects. Clonally expanded mtDNA deletions and focal mtDNA depletion may contribute towards the development of age\u2010related postural abnormalities.<\/jats:p>","DOI":"10.1111\/j.1365-2990.2012.01290.x","type":"journal-article","created":{"date-parts":[[2012,7,5]],"date-time":"2012-07-05T14:23:26Z","timestamp":1341498206000},"page":"377-389","source":"Crossref","is-referenced-by-count":19,"title":["Mitochondrial DNA deletions and depletion within paraspinal muscles"],"prefix":"10.1111","volume":"39","author":[{"given":"G. R.","family":"Campbell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Reeve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Ziabreva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. M.","family":"Polvikoski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. W.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Reynolds","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. M.","family":"Turnbull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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