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Amoebic trophozoites induced marked degradation of paxillin, Cas, vimentin, vinculin and talin, as well as \u03b1\u2010 or \u03b2\u2010spectrin, in Jurkat T cells. The cleavage effects of <jats:italic>E.\u00a0histolytica<\/jats:italic> were strongly retarded by pretreatment with a calpain inhibitor, but not with a pan\u2010caspase inhibitor. In addition, calpain knockdown with siRNA in Jurkat T cells effectively inhibited <jats:italic>E.\u00a0histolytica<\/jats:italic>\u2010induced PARP, paxillin, \u03b1\u2010spectrin, \u03b2\u2010spectrin and talin degradation, as compared to scrambled siRNA. These results suggest that calpain plays a crucial role in the cleavage of cytoskeletal proteins during cell death induced by <jats:italic>E.\u00a0histolytica<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-3024.2011.01290.x","type":"journal-article","created":{"date-parts":[[2011,3,23]],"date-time":"2011-03-23T08:19:39Z","timestamp":1300868379000},"page":"349-356","source":"Crossref","is-referenced-by-count":9,"title":["Calpain mediates degradation of cytoskeletal proteins during Jurkat T\u2010cell death induced by <i>Entamoeba histolytica<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Y. A.","family":"LEE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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