{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:42:05Z","timestamp":1767163325147,"version":"build-2238731810"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T00:00:00Z","timestamp":1330992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    American cutaneous leishmaniasis (ACL) has different clinical manifestations and these manifestations are dependent on the immunological status of the host. As CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and their mediators play a fundamental role in the host response to\n                    <jats:italic>Leishmania<\/jats:italic>\n                    and there is also a search for antigenic molecules to be used as future vaccines and tools for prognostic tests, this study characterized ACL patients\u2019 immune response after stimulation with soluble and insoluble fractions of\n                    <jats:italic>L.\u00a0(V.) braziliensis<\/jats:italic>\n                    . We demonstrated a prevailing production of the Th2 cytokines, IL\u20104 and IL\u201010 and a specific production of IFN\u2010\u03b3 and TNF\u2010\u03b1 in patients before treatment. There was also a predominance of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells and a small percentage CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. The insoluble antigenic fraction primarily stimulated CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, while the soluble antigenic fraction showed a mixed profile, with CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells being the main responsible for Th2 cytokines and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells for Th1 cytokines. Therefore, our results showed that a down\u2010modulation of the Th1 type of response occurs in the initial phase of\n                    <jats:italic>L.\u00a0braziliensis<\/jats:italic>\n                    disease, being the antigenic fractions capable of stimulating a specific immune response.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-3024.2012.01351.x","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T11:31:02Z","timestamp":1331033462000},"page":"236-239","source":"Crossref","is-referenced-by-count":11,"title":["Antigenic fractions of\n                    <i>Leishmania (Viannia) braziliensis<\/i>\n                    : the immune response characterization of patients at the initial phase of disease"],"prefix":"10.1111","volume":"34","author":[{"given":"M. C. A.","family":"BRELAZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"De OLIVEIRA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. F.","family":"De ALMEIDA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"De ASSIS SOUZA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c2. C. R.","family":"MEDEIROS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E. F.","family":"De BRITO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. R. A.","family":"PEREIRA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.02995.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2009.02361.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762001000100010"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-6-75"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20291"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.9.6139"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.01.007"},{"key":"e_1_2_6_9_2","first-page":"251","article-title":"T\u2010Cell\u2010mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long\u2010term evaluation after therapy","volume":"9","author":"Da\u2010Cruz AM","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0100"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(01)90168-3"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.2.303-313.2001"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.04.028"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2741\/2106"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3232-3239.2001"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1999.00554.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01398-08"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3024.2012.01351.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3024.2012.01351.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T16:56:48Z","timestamp":1698685008000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3024.2012.01351.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,6]]},"references-count":16,"aliases":["10.1111\/j.1365-3024.2011.01351.x"],"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1365-3024.2012.01351.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3024.2012.01351.x","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,6]]}}}