{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:59:48Z","timestamp":1760853588647},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T00:00:00Z","timestamp":1336694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The need to develop an effective vaccine against leishmaniasis to prevent the 2\u2003million new cases each year led to the search for antigens able to elicit protection against infection with <jats:italic>Leishmania<\/jats:italic>. In this study, we have characterized a parasite\u2010specific protein of <jats:italic>Leishmania infantum<\/jats:italic> named thiol\u2010dependent reductase 1 (TDR1). The protein is present in both life cycle stages of <jats:italic>L.\u00a0infantum<\/jats:italic> with a notable higher expression in the amastigote forms, suggesting a role in the interaction between the parasite and the mammalian host. Thiol\u2010dependent reductase 1 is localized in the cytosol, although we were able to detect the protein in the culture medium of both promastigotes and axenic amastigotes, and consequently, TDR1 is considered an excreted\/secreted molecule of the parasite. Therefore, we have evaluated the potential of TDR1 recombinant protein to protect against experimental challenge with <jats:italic>L.\u00a0infantum<\/jats:italic> parasites using a murine model. Despite a reduction in spleen parasite load in the chronic phase of disease, TDR1 administration was not effective in the protection of Balb\/c mice against visceral leishmaniasis and thus TDR1 do not have a crucial role in the modulation of mammalian host immune response, as observed with its protein counterpart Tc52 of <jats:italic>Trypanosoma cruzi<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-3024.2012.01361.x","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T18:39:26Z","timestamp":1331750366000},"page":"345-350","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of <i>Leishmania infantum<\/i> thiol\u2010dependent reductase 1 and evaluation of its potential to induce immune protection"],"prefix":"10.1111","volume":"34","author":[{"given":"A. M.","family":"SILVA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"TAVARES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"SILVESTRE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"OUAISSI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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