{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:23:39Z","timestamp":1791012219568,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,7,12]],"date-time":"2011-07-12T00:00:00Z","timestamp":1310428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Current studies, particularly in <jats:italic>Arabidopsis<\/jats:italic>, have demonstrated that mutants deficient in cytosolic ascorbate peroxidases (APXs) are susceptible to the oxidative damage induced by abiotic stress. In contrast, we demonstrate here that rice mutants double silenced for cytosolic APXs (APx1\/2s) up\u2010regulated other peroxidases, making the mutants able to cope with abiotic stress, such as salt, heat, high light and methyl viologen, similar to non\u2010transformed (NT) plants. The APx1\/2s mutants exhibited an altered redox homeostasis, as indicated by increased levels of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and ascorbate and glutathione redox states. Both mutant and NT plants exhibited similar photosynthesis (CO<jats:sub>2<\/jats:sub> assimilation and photochemical efficiency) under both normal and stress conditions. Overall, the antioxidative compensatory mechanism displayed by the mutants was associated with increased expression of <jats:italic>OsGpx<\/jats:italic> genes, which resulted in higher glutathione peroxidase (GPX) activity in the cytosolic and chloroplastic fractions. The transcript levels of <jats:italic>OsCatA<\/jats:italic> and <jats:italic>OsCatB<\/jats:italic> and the activities of catalase (CAT) and guaiacol peroxidase (GPOD; type III peroxidases) were also up\u2010regulated. None of the six studied isoforms of <jats:italic>OsApx<\/jats:italic> were up\u2010regulated under normal growth conditions. Therefore, the deficiency in cytosolic APXs was effectively compensated for by up\u2010regulation of other peroxidases. We propose that signalling mechanisms triggered in rice mutants could be distinct from those proposed for <jats:italic>Arabidopsis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-3040.2011.02366.x","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T12:00:10Z","timestamp":1306929610000},"page":"1705-1722","source":"Crossref","is-referenced-by-count":118,"title":["Role of peroxidases in the compensation of cytosolic ascorbate peroxidase knockdown in rice plants under abiotic stress"],"prefix":"10.1111","volume":"34","author":[{"given":"AURENIVIA","family":"BONIFACIO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARCIO O.","family":"MARTINS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CAROLINA W.","family":"RIBEIRO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ADILTON V.","family":"FONTENELE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FABRICIO E. L.","family":"CARVALHO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00c1RCIA","family":"MARGIS\u2010PINHEIRO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOAQUIM A. G.","family":"SILVEIRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,7,12]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"497","article-title":"Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants","volume":"35","author":"Amako K.","year":"1994","journal-title":"Plant Cell Physiology"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1992.tb04728.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2004.02.008"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"5144","DOI":"10.1016\/S0021-9258(19)41289-1","article-title":"Purification and properties of human erythrocyte glutathione peroxidase","volume":"250","author":"Awasthi Y.C.","year":"1975","journal-title":"The Journal of Biological Chemistry"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(66)09069-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90370-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein\u2010dye binding","volume":"722","author":"Bradford M.M.","year":"1976","journal-title":"Analytical Biochemistry"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.59.3.411"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1991.tb00121.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2004.01139.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2007.11.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.135566"},{"key":"e_1_2_6_15_1","first-page":"1","volume-title":"Photosynthesis Research Protocols","author":"Cuello J.","year":"2007"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.026971"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9020378"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-8137.2000.00667.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3054.2003.00223.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.033589"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2177"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.166181"},{"key":"e_1_2_6_23_1","first-page":"455","article-title":"Photorespiratory metabolism: genes, mutants, energetics, and redox signaling","volume":"60","author":"Foyer C.H.","year":"2009","journal-title":"Annual Review of Plant Physiology"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.59.2.309"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90139-6"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.84.2.450"},{"key":"e_1_2_6_27_1","first-page":"32","volume-title":"The Water\u2010Culture Method for Growing Plants Without Soil","author":"Hoagland D.R.","year":"1950"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2009.02.001"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1127"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M806337200"},{"key":"e_1_2_6_31_1","first-page":"1","article-title":"Ascorbate peroxidase\u2010related (APx\u2010R) is a new heme\u2010containing protein functionally associated with ascorbate peroxidase but evolutionarily divergent","volume":"190","author":"Lazzarotto F.","year":"2011","journal-title":"New Phytologist"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(00)00152-4"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-007-0395-7"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06542.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcp177"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2003.10.001"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.101436"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2009.02041.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(02)02312-9"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470988565.ch4"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2004.08.009"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.58.032806.103946"},{"key":"e_1_2_6_44_1","first-page":"1068","article-title":"Hydrogen peroxide is scavenged by ascorbate\u2010specific peroxidase in spinach chloroplasts","volume":"22","author":"Nakano Y.","year":"1981","journal-title":"Plant Cell Physiology"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.089458"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcf096"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.078469"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01715.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)01423-4"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2002.01427.x"},{"key":"e_1_2_6_51_1","first-page":"1683","article-title":"When defense pathways collide. The response of Arabidopsis to a combination of drought and heat Stress","volume":"134","author":"Rizhsky L.","year":"2004","journal-title":"Stress: The International Journal on the Biology of Stress"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2010.01.003"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/53.372.1305"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2010.03.005"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2007_3267"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHYT.0000047805.08470.e3"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-004-2666-z"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-0214-8"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.03.016"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3040.2011.02366.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3040.2011.02366.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T10:07:38Z","timestamp":1698746858000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3040.2011.02366.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,12]]},"references-count":60,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["10.1111\/j.1365-3040.2011.02366.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3040.2011.02366.x","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7,12]]}}}