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We observed a biphasic [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> signature in root cells that was distinct from that in epicotyl and leaf cells, which showed a monophasic response after HS. Treatment with Ca<jats:sup>2+<\/jats:sup> and A23187 generated an intense and sustained increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> in response to HS. Conversely, treatment with Ca<jats:sup>2+<\/jats:sup> chelator, L\u2010type Ca<jats:sup>2+<\/jats:sup> channel blocker and CaM antagonist, but not intracellular Ca<jats:sup>2+<\/jats:sup> release inhibitor, strongly inhibited the increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub>. HS combined with Ca<jats:sup>2+<\/jats:sup> and A23187 accelerated the expression of <jats:italic>OsCaM1\u20101<\/jats:italic> and <jats:italic>sHSPC\/N<\/jats:italic> genes, which suggests that the HS\u2010induced apoplastic Ca<jats:sup>2+<\/jats:sup> influx is responsible for the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> response and downstream HS signalling. In addition, the biphasic response of OsCaM1\u20101 in the nucleus followed the Ca<jats:sup>2+<\/jats:sup> signature, which may provide the information necessary to direct HS\u2010related gene expression. Overexpression of <jats:italic>OsCaM1\u20101<\/jats:italic> induced the expression of Ca<jats:sup>2+<\/jats:sup>\/HS\u2010related <jats:italic>AtCBK3<\/jats:italic>, <jats:italic>AtPP7<\/jats:italic>, <jats:italic>AtHSF<\/jats:italic> and <jats:italic>AtHSP<\/jats:italic> at a non\u2010inducing temperature and enhanced intrinsic thermotolerance in transgenic <jats:italic>Arabidopsis<\/jats:italic>. Therefore, HS\u2010triggered rapid increases in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub>, together with <jats:italic>OsCaM1\u20101<\/jats:italic> expression and its nuclear localization, are important in mediating downstream HS\u2010related gene expression for the acquisition of thermotolerance in rice.<\/jats:p>","DOI":"10.1111\/j.1365-3040.2012.02508.x","type":"journal-article","created":{"date-parts":[[2012,3,19]],"date-time":"2012-03-19T15:20:58Z","timestamp":1332170458000},"page":"1543-1557","source":"Crossref","is-referenced-by-count":84,"title":["Heat shock\u2010induced biphasic Ca<sup>2+<\/sup> signature and OsCaM1\u20101 nuclear localization mediate downstream signalling in acquisition of thermotolerance in rice (<i>Oryza sativa<\/i> L.)"],"prefix":"10.1111","volume":"35","author":[{"given":"HUI\u2010CHEN","family":"WU","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DAN\u2010LI","family":"LUO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FLORENCE","family":"VIGNOLS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TSUNG\u2010LUO","family":"JINN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02712120"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-7-4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.56.032604.144224"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02272.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.4.1590-1597.1990"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.074898"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.091322"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1999.00411.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(91)90117-Q"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4426"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-004-2891-y"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.116.1.429"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-004-5182-z"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2001.01066.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3513"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.5.8.12404"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.151225"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.179036"},{"key":"e_1_2_6_21_1","first-page":"1055","article-title":"Immunological kinship of class I low molecular weight heat shock proteins and thermostabilization of soluble proteins in vitro among plants","volume":"34","author":"Jinn T.L.","year":"1993","journal-title":"Plant and Cell Physiology"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1104\/pp.114.2.429","article-title":"Tissue\u2010type\u2010specific heat\u2010shock response and immunolocalization of class I low\u2010molecular\u2010weight heat\u2010shock proteins in soybean","volume":"114","author":"Jinn T.L.","year":"1997","journal-title":"Plant Physiology"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.042713"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/ssn091"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2007.04.011"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3054.2003.00185.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010320"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.005579"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00562-K"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1016\/S0021-9258(18)53924-7","article-title":"A second groEL\u2010like gene, organized in a groESL operon is present in the genome of Synechocystis sp. 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