{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T00:39:05Z","timestamp":1775263145798,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,4,22]],"date-time":"2005-04-22T00:00:00Z","timestamp":1114128000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immune modulatory properties of recombinant antigens Kinetoplasmid membrane protein\u201011 (KMP11) and <jats:italic>Leishmania<\/jats:italic> homologue of receptors for activated C kinase (LACK) in cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML) patients were evaluated. The mean levels of interferon\u2010\u03b3 (IFN\u2010\u03b3) in soluble leishmania antigen (SLA) stimulated peripheral blood mononuclear cells (PBMC) of ML and CL patients were 5625\u2003\u00b1\u20032333\u2003pg\/ml and 4422\u2003\u00b1\u20033665\u2003pg\/ml, respectively. IFN\u2010\u03b3 was not detected in cultures stimulated with KMP11 or LACK. Interleukin\u201010 (IL\u201010) concentration in SLA, KMP11 and LACK\u2010stimulated PBMC of ML patients was 13\u2003\u00b1\u200312\u2003pg\/ml, 285\u2003\u00b1\u2003388\u2003pg\/ml and 802\u2003\u00b1\u2003483\u2003pg\/ml, respectively. Addition of KMP11 or LACK to SLA\u2010stimulated PBMC of CL and ML patients enhanced IL\u201010 production (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Addition of KMP11 decreased IFN\u2010\u03b3 levels by 52% in CL patients and by 19% in ML patients. Addition of LACK to SLA\u2010stimulated cultures decreased IFN\u2010\u03b3 levels by 58% in CL patients and by 30% in ML patients. Neutralization of IL\u201010 abrogated the downregulatory effect of LACK and KMP11. The modulatory properties of LACK and KMP11 are due to induction of IL\u201010 production and may be helpful for attenuating chronic inflammatory diseases. However, in some clinical conditions, as demonstrated for ML, these molecules are not able to suppress the IFN\u2010\u03b3 response, even inducing IL\u201010 production.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2005.01581.x","type":"journal-article","created":{"date-parts":[[2005,4,22]],"date-time":"2005-04-22T13:25:50Z","timestamp":1114176350000},"page":"337-342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Effect of LACK and KMP11 on IFN\u2010\u03b3 Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients"],"prefix":"10.1111","volume":"61","author":[{"given":"L. 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