{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T11:43:49Z","timestamp":1749555829113},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,2,27]],"date-time":"2006-02-27T00:00:00Z","timestamp":1140998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p> <jats:italic>Clonorchis sinensis<\/jats:italic>, the Chinese liver fluke, resides chronically in the biliary tract, and fatty acid\u2010binding protein (FABP) is known to play an important role in the intracellular transport of long\u2010chain fatty acids obtained from the host. Although FABP has stimulated considerable interest as a vaccine candidate, the nature of <jats:italic>C. sinensis<\/jats:italic> FABP (CsFABP) remains unclear. We investigated the immunogenicity and protective efficacy of a DNA vaccine encoding CsFABP. The intradermal injection of plasmid DNA carrying the <jats:italic>CsFABP<\/jats:italic> gene (pcDNA3.1\u2010FABP) into Sprague\u2013Dawley (SD) rats induced both humoural and cellular immune responses. Animals injected with pcDNA3.1\u2010FABP developed FABP\u2010specific antibody, which is dominance of IgG2a in sera. In addition, the DNA vaccine elicited the production of IFN\u2010\u03b3, but not the production of IL\u20104 in spleen cells stimulated with recombinant FABP. Moreover, pcDNA3.1\u2010FABP induced a significant level of protection, decreased worm burden (40.9%, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) in SD rats against <jats:italic>C. sinensis<\/jats:italic> metacerariae challenge. These results suggest that pcDNA3.1\u2010FABP induces a typical T helper\u20101\u2010dominated immune response and it is a good candidate for use in future clonorchiasis vaccination studies.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2006.01721.x","type":"journal-article","created":{"date-parts":[[2006,2,27]],"date-time":"2006-02-27T09:34:10Z","timestamp":1141032850000},"page":"169-176","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A DNA Vaccine Encoding a Fatty Acid\u2010Binding Protein of <i>Clonorchis sinensis<\/i> Induces Protective Immune Response in Sprague\u2013Dawley Rats"],"prefix":"10.1111","volume":"63","author":[{"given":"J.\u2010S.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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