{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:12:18Z","timestamp":1770480738365,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T00:00:00Z","timestamp":1166054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Understanding the specificity of antibody response to <jats:italic>Neisseria meningitidis<\/jats:italic> serogroup B (Men B) is a key requirement for the development of an effective vaccine. This study was designed to investigate the antigen specificity of murine IgG1 and IgG2b antibodies induced by different primary immunization schedules and the booster dose with the Cuban Men B vaccine. Immunoblotting analyses were performed using outer membrane vesicles (OMV) from the vaccine strain (B:4,7:P1.19,15). IgG subclasses binding to PorA, PorB and RmpM were determined by digital scanning of the immunoreactive bands. Bactericidal antibody response after vaccination was also evaluated. The results indicated that IgG2b anti\u2010PorA was the main antibody response induced by two doses of the vaccine. A primary series of three doses was found important for increasing IgG2b as well as IgG1 to PorB and RmpM. The fourth dose favoured the recognition of RmpM as detected by the increase of specific IgG1 and IgG2b. IgG subclasses anti\u2010PorA did not change significantly if animals received two, three or four doses of the vaccine during the primary immunization or after the booster dose for all vaccine groups. The booster response to PorB and RmpM of groups BC2 and BC3 showed a significant increase in IgG2b levels compared with the primary response. However, the recall and the primary response of group BC4 were similar, suggesting a saturated dose\u2013effect response after four doses of vaccine. The same was seen for bactericidal antibody response when human complement source was used in the assay.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2006.01829.x","type":"journal-article","created":{"date-parts":[[2006,12,15]],"date-time":"2006-12-15T01:16:47Z","timestamp":1166145407000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential Capacities of Outer Membrane Proteins from <i>Neisseria meningitidis<\/i> B to Prime the Murine Immune System after Vaccination"],"prefix":"10.1111","volume":"65","author":[{"given":"F. C.","family":"Silva Junior","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. A. C.","family":"Gioia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. C.","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. E.","family":"Frasch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. G.","family":"Milagres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00552-1"},{"key":"e_1_2_6_3_2","first-page":"1730","article-title":"Antibody to genome\u2010derived neisserial antigen 2132, a Neisseria meningitidis candidate vaccine, confers protection against bacteremia in the absence of complement\u2010mediated bactericidal activity","volume":"188","author":"Welsch JA","year":"2003","journal-title":"J Pediatr Infect Dis"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.9.5606"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.01.058"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.01.063"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200111000-00010"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)93086-3"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2004.11.013"},{"key":"e_1_2_6_10_2","first-page":"129","volume-title":"Neisseria 1990","author":"Sierra VGG","year":"1991"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1128\/jb.141.1.169-176.1980","article-title":"Chemical analysis of major outer membrane proteins of Neisseria meningitidis: comparison of serotypes 2 and 11","volume":"141","author":"Tsai CM","year":"1980","journal-title":"J Bacteriol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"4419","DOI":"10.1128\/iai.62.10.4419-4424.1994","article-title":"Immune response of Brazilian children to a Neisseria meningitidis serogroup B outer membrane protein vaccine: comparison with efficacy","volume":"62","author":"Milagres LG","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00245-3"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/334255a0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000235071"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8119-8129.2005"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4502-4508.2001"},{"key":"e_1_2_6_18_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1128\/IAI.66.3.959-965.1998","article-title":"Human T\u2010cell responses after vaccination with the Norwegian group B menincococcal outer membrane vesicle vaccine","volume":"66","author":"N\u00e6ss LM","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_19_2","doi-asserted-by":"crossref","first-page":"4642","DOI":"10.1128\/iai.63.12.4642-4652.1995","article-title":"Human antibody responses to meningococcal outer membrane antigens after three doses of the Norwegian group B meningococcal vaccine","volume":"63","author":"Rosenqvist E","year":"1995","journal-title":"Infect Immun"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(91)90041-8"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1267-1276.1999"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3083.2006.01829.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3083.2006.01829.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:56:56Z","timestamp":1696899416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3083.2006.01829.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,14]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1111\/j.1365-3083.2006.01829.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3083.2006.01829.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12,14]]},"assertion":[{"value":"2006-12-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}