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In this study, we examined the response of na\u00efve human peripheral blood mononuclear cells (nPBMC) to stimulation with <jats:italic>Schistosoma mansoni<\/jats:italic> soluble egg antigen (SEA) using a priming <jats:italic>in vitro<\/jats:italic> (PIV) assay. We found that SEA induced a pronounced CD4<jats:sup>+<\/jats:sup> T\u2010helper cell response based on cytokine secretion and phenotyping markers. SEA\u2010stimulated nPBMC (SEA cells) at day 7 post\u2010priming and after the first recall consisted predominantly of Th0\u2010like CD4<jats:sup>+<\/jats:sup> T cells. Following the second recall, the majority of donor (10\/12) responses were Th2\u2010like. The cell population consisted of approximately 64% CD4<jats:sup>+<\/jats:sup>, 17% CD8<jats:sup>+high<\/jats:sup>, 12% CD19<jats:sup>+<\/jats:sup>, and 7% CD23<jats:sup>+<\/jats:sup> cells. The CD4<jats:sup>+<\/jats:sup> population also expressed HLA\u2010DR<jats:sup>+<\/jats:sup>, CD54<jats:sup>+<\/jats:sup>, CD45RO<jats:sup>+<\/jats:sup> and CD25<jats:sup>+<\/jats:sup> whereas the CD19<jats:sup>+<\/jats:sup> cells expressed CD80 and CD86. Following priming, we detected high levels of IL\u20106, IFN\u2010\u03b3, IL\u201012p40, IL\u201010 and IL\u20105. Upon restimulation, SEA cells secreted IL\u20105 and high levels of IL\u201010, typical of a Th2\u2010like response. The data presented herein shows that the majority of na\u00efve donor dendritic cells, following stimulation with SEA, prime and clonally expand SEA\u2010specific T cells towards a Th2\u2010type response. However, two donors responded with an atypical response, producing IFN\u2010\u03b3 coincident with low levels of IL\u201010. 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