{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:47:56Z","timestamp":1761662876644},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,7,6]],"date-time":"2010-07-06T00:00:00Z","timestamp":1278374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Early secretory antigen target 6 (ESAT\u20106) is a dominant target for cell\u2010mediated immunity in the early phase of tuberculosis (TB) in patients with TB, causing T\u2010cell proliferation and gamma interferon (IFN\u2010\u03b3) production, which has been considered to be a protective antigen that can be used for future vaccine development. Ag85A is the most essential component for bacterial survival within macrophages and has been used in numerous vaccine preparations, which can induce strong cellular immune responses. In this study, we constructed a new recombinant bacilli Calmette<jats:bold>\u2010<\/jats:bold>Gu\u00e9rin (BCG) strain (rBCG\u2010AE) that could express fusion protein Ag85A\u2010ESAT\u20106 of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> and evaluated its immunogenicity in BALB\/c mice. There was no evidence for increased virulence of this rBCG. Our experiments illustrated that the rBCG\u2010AE was able to induce higher titer of antibody and elicit more long\u2010lasting and stronger Th1 type cellular immune responses than the parental BCG strain, or rBCG\u2010A (expressing Ag85A) strain, or rBCG\u2010E (expressing ESAT\u20106) strain, which are characterized by the strong antibody response, the proliferation rate of splenocytes, the ratio of CD4<jats:sup>+<\/jats:sup> T and CD8<jats:sup>+<\/jats:sup> T cells stimulated by tuberculin\u2010purified protein derivative and elevated levels of IFN\u2010\u03b3 in antigen\u2010stimulated splenocyte cultures. The results show that rBCG\u2010AE is an improved TB vaccine for further study.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2010.02444.x","type":"journal-article","created":{"date-parts":[[2010,7,7]],"date-time":"2010-07-07T08:26:31Z","timestamp":1278491191000},"page":"332-338","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Improved Immunogenicity of Recombinant <i>Mycobacterium bovis<\/i> Bacillus Calmette\u2010Gu\u00e9rin Strains Expressing Fusion Protein Ag85A\u2010ESAT\u20106 of <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1111","volume":"72","author":[{"given":"Y. H.","family":"Deng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. L.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Global Tuberculosis Control: Surbeillance, Planning, Financing","author":"WHO","year":"2008"},{"key":"e_1_2_6_3_2","first-page":"171","article-title":"Emerging epidemic of drug\u2010resistant tuberculosis in Europe, Russia, China, South America and Asia: current status and global perspectives","volume":"16","author":"Migliori GB","year":"2010","journal-title":"Curr Opin Pulm Med"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1293.2008.00567.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-9792(02)00071-9"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.08.009"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.03.029"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.05.048"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.02.094"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2007.11.016"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.11.7948"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00322.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.1.16-22.1996"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5419.1520"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/9.2.227"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.767-778.2000"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1780"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/351456a0"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1656-1661.2003"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.483-493.2003"},{"key":"e_1_2_6_21_2","first-page":"668s","article-title":"Functions of T\u2010cell subsets and cytokines in mycobacterial infections","volume":"20","author":"Munk ME","year":"1995","journal-title":"Eur Respir J Suppl"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.4296"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2008.09.008"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3083.2010.02444.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3083.2010.02444.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T17:24:37Z","timestamp":1697304277000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3083.2010.02444.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1111\/j.1365-3083.2010.02444.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3083.2010.02444.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]},"assertion":[{"value":"2010-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}