{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:22:14Z","timestamp":1763036534568},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T00:00:00Z","timestamp":1297123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>IL\u201017\u2010producing T cells (Th17 cells) are believed to contribute to local inflammation and joint damage in rheumatoid arthritis (RA). Limited data exist on Th17 cells located within the inflamed synovial tissue (ST) of patients with RA. Here, we aimed to generate polyclonal T cell lines (TCLs) from the RA ST and assess their cytokine production, including the effects of exogenous IL\u201015 on IL\u201017 production <jats:italic>in vitro<\/jats:italic>. For five patients with RA, polyclonal TCLs were established from ST obtained by joint surgery. Synovial TCLs were expanded and stimulated by anti\u2010CD3\/CD28 microbeads and exogenous cytokines. Cytokine production was assessed by culture supernatant analyses and intracellular flow cytometry, and TCLs were sorted based on their surface expression of CCR6. In addition to IL\u201017, we detected IL\u20106, IL\u201010, IFN\u2010\u03b3 and TNF\u2010\u03b1 in the synovial TCL culture supernatants. Exogenous IL\u201015 increased the production of IL\u201017 as well as the other cytokines except IFN\u2010\u03b3. For IL\u201017, this effect was more pronounced after prolonged culture times. Intracellular flow cytometry confirmed the presence of IL\u201017<jats:sup>+<\/jats:sup> and IL\u201017<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> T cells in the TCLs. IL\u201017<jats:sup>+<\/jats:sup> and IL\u201017<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup> T cells were enriched in the CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup> population. In conclusion, Th17 cells can be detected after polyclonal expansion and stimulation of RA synovial TCLs generated by joint surgery. The Th17 cells from the RA ST were enriched in the CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup> population, and they were sensitive to exogenous IL\u201015. Th17 cells present within the synovial compartment may contribute to the RA pathogenesis and local joint damage.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2010.02498.x","type":"journal-article","created":{"date-parts":[[2010,11,20]],"date-time":"2010-11-20T14:49:18Z","timestamp":1290264558000},"page":"243-249","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Interleukin\u201015 Induces Interleukin\u201017 Production by Synovial T Cell Lines from Patients with Rheumatoid Arthritis"],"prefix":"10.1111","volume":"73","author":[{"given":"E. H.","family":"Halvorsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Str\u00f8nen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. B.","family":"Hammer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. L.","family":"Goll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M.","family":"Sollid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8.","family":"Molberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,2,8]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0707449"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1478"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5703"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.5.2832"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1733"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071397"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21749"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199905)42:5<963::AID-ANR15>3.0.CO;2-E"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24687"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808144106"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.3.1246"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.11.7423"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070663"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.23291"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1467"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.7948"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.1.214"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.080341"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kep293"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903566"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0698-713"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2006.02.006"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0296-175"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0297-189"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2010.01.010"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1497"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2009.02361.x"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/10799900152434376"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.25064"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keg413"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3083.2010.02498.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3083.2010.02498.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T13:49:29Z","timestamp":1698760169000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3083.2010.02498.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,8]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1111\/j.1365-3083.2010.02498.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3083.2010.02498.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,8]]},"assertion":[{"value":"2011-02-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}