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We determined reference values for B\u2010lymphocyte subpopulations in healthy children with the statistical method of tolerance intervals that deals far better with the relatively small numbers tested, and compared these to the cut\u2010off values used in the currently used EUROclass classification for common variable immunodeficiency disorders (CVID) in children. CVID is a heterogeneous group of primary immunodeficiency diseases characterized by low serum immunoglobulin levels and inadequate response to vaccination. Disease\u2010modifying heterozygous amino acid substitutions in TACI are found in around \u00b110% of CVID patients. Interestingly, we found that age is the primary determinant of TACI\u2010expression on B\u2010lymphocytes, independent of switched memory B\u2010lymphocyte numbers. Immunophenotyping of B\u2010lymphocyte subpopulations is increasingly used to classify patients with CVID into subgroups with different clinical prognosis according to the composition of their B\u2010lymphocyte compartment. These classifications were mainly developed with data obtained in adults. Because of the maturing paediatric immune system, they may not be equally applicable in children: our and other age\u2010matched reference values show great changes in the composition of the B\u2010lymphocyte compartment during development. Although the greatest changes in B\u2010lymphocyte subpopulations occur below the age of 2\u2003years, when the diagnosis of CVID cannot yet be made, it is likely that a classification developed in adults cannot be used to classify the prognosis of children.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2011.02609.x","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T21:37:12Z","timestamp":1312493832000},"page":"502-510","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Age\u2010matched Reference Values for B\u2010lymphocyte Subpopulations and CVID Classifications in Children"],"prefix":"10.1111","volume":"74","author":[{"given":"E. J. H.","family":"Schatorj\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. F. 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