{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T01:37:36Z","timestamp":1717810656669},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,11,14]],"date-time":"2011-11-14T00:00:00Z","timestamp":1321228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Shiga toxin 1 produced by enterohaemorrhagic <jats:italic>Escherichia coli<\/jats:italic> is an AB<jats:sub>5<\/jats:sub> toxin that is involved in the life\u2010threatening haemolytic\u2010uraemic syndrome. The B subunits (Stx1B) are cell\u2010binding subunits. We previously established mouse hybridoma cell line producing IgA and IgG monoclonal antibodies (mAbs) against Stx1B. Here, we cloned cDNAs encoding each of the heavy, light and joining (J) chains from the hybridoma cell lines by means of the 5\u2032 rapid amplification of cDNA ends (RACE) PCR method. Upon assignment of the variable regions of the heavy and light chains to known germline sequences, we found substantial somatic hypermutation in the complementarity\u2010determining regions in both the IgA and IgG mAbs. We also established a hybrid\u2010IgG\/IgA heavy chain having variable regions of the IgG mAb by means of recombinant PCR methods. Upon transient expression of the hybrid\u2010IgG\/IgA heavy, IgG\u2010associated light and J chains in COS\u20101 cells, the translated dimeric hybrid\u2010IgG\/IgA bound to immobilized Stx1B, as revealed on ELISA. The production of dimeric hybrid\u2010IgG\/IgA was revealed on immunoblot analysis. The dimeric hybrid\u2010IgG\/IgA inhibited the binding of digoxigenin\u2010conjugated Stx1B to natural ligands (CD77) displayed on Burkitt\u2019s lymphoma cell line Ramos. These results indicate that the replacement of variable regions resulted in the production of more useful recombinant dimeric IgA against Stx1B.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2011.02617.x","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T03:50:20Z","timestamp":1314849020000},"page":"574-584","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishment of Recombinant Hybrid\u2010IgG\u200a\/\u200aIgA Immunoglobulin Specific for Shiga Toxin"],"prefix":"10.1111","volume":"74","author":[{"given":"Y.","family":"Tobisawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Maruyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Tanikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kurohane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro818"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.142"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036079"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-77238-2_4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.450"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2568"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(90)90050-Z"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00805.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/355748a0"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47490-X"},{"key":"e_1_2_6_13_2","first-page":"5314","article-title":"Apoptosis induced in Burkitt\u2019s lymphoma cells via Gb3\/CD77, a glycolipid antigen","volume":"53","author":"Mangeney M","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199910)29:10<3236::AID-IMMU3236>3.0.CO;2-T"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01373.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.18.3.775-779.1977"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1877"},{"key":"e_1_2_6_18_2","doi-asserted-by":"crossref","first-page":"284","DOI":"10.4049\/jimmunol.114.1_Part_2.284","article-title":"Increased resistance of immunoglobulin A dimers to proteolytic degradation after binding of secretory component","volume":"114","author":"Lindh E","year":"1975","journal-title":"J Immunol"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.1998.tb01645.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1601489"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.177.4050.697"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-46830-1_7"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2003.01700.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.05.007"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2008.02153.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.889-895.2004"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007107425891"},{"key":"e_1_2_6_28_2","first-page":"177","volume-title":"PCR Protocols; 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