{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T04:39:17Z","timestamp":1769747957088,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,8,22]],"date-time":"2012-08-22T00:00:00Z","timestamp":1345593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate vaccine, the Bacille Calmette\u2010Gu\u00e9rin (BCG), is urgently needed. BCG has proven to be an effective recombinant delivery vehicle for foreign antigens because of its ability to induce long\u2010lived specific humoral and cellular immunity. Experimental evidences have revealed that Ag85B, ESAT\u20106 and Rv2608 are important immunodominant antigens of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> and are all promising vaccine candidate molecules. In this study, we have constructed a novel recombinant BCG (rBCG) expressing fusion protein Ag85B\u2010ESAT6\u2010Rv2608 and evaluated the immunogenicity of rBCG in C57BL\/6 mice. Results show there is strong TB\u2010specific CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T lymphocytes proliferative response in mice immunized with rBCG vaccine, especially the cytotoxic CD8<jats:sup>+<\/jats:sup> T cells playing an important role in protection against TB. And rBCG immunization has induced a significantly strong Th1 immune response, characterized by the increased ratio of IgG2b\/IgG1. Results also show that rBCG immunization could increase the secretion of Th1 cytokines such as TNF\u2010\u03b1 and IL\u20102 and could decrease the secretion of Th2 cytokine IL\u201010. Moreover, it was shown that rBCG immunization induced a strong humoral response in mice, characterized by the elevated IgG titre. Therefore, we conclude that this rBCG immunization could increase both cellular immune response and antigen\u2010specific humoral response significantly as compared to BCG immunization in mice. The above results illustrated that rBCG::Ag85B\u2010ESAT6\u2010Rv2608 is a potential candidate against <jats:italic>M.\u00a0tuberculosis<\/jats:italic> for further study.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2012.02726.x","type":"journal-article","created":{"date-parts":[[2012,6,5]],"date-time":"2012-06-05T05:24:02Z","timestamp":1338873842000},"page":"271-277","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Novel Recombinant BCG Coexpressing Ag85B, ESAT\u20106 and Rv2608 Elicits Significantly Enhanced Cellular Immune and Antibody Responses in C57BL\/6 Mice"],"prefix":"10.1111","volume":"76","author":[{"given":"Y.","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,22]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"U.S. Global Health Policy","author":"The Henry J Kaiser Family Foundation","year":"2010"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199806043382301"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.011"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818357.ch31"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91074-K"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)92348-9"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.56.4.648-661.1992"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.250480397"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1672-1679.2003"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.791-795.2000"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.7.3359"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-10-2919"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.07.026"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.05.025"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1086\/423938","article-title":"Regions of high antigenicity within the hypothetical PPE major polymorphic tandem repeat open\u2010reading frame, Rv2608, show a differential humoral response and a low T cell response in various categories of patients with tuberculosis","volume":"190","author":"Chakhaiyar P","year":"2004","journal-title":"J Infect"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.11.7948"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2007.02048.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00232.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1656-1661.2003"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)87499-3"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.07.036"},{"key":"e_1_2_6_23_2","doi-asserted-by":"crossref","first-page":"5969","DOI":"10.4049\/jimmunol.154.11.5969","article-title":"Cytokine function during mycobacterial and schistosomal antigen\u2010induced pulmonary granuloma formation. Local and regional participation of IFN\u2010gamma, IL\u201010, and TNF","volume":"154","author":"Chensue SW","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxf050"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.9.5407"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"5223","DOI":"10.1128\/IAI.67.10.5223-5230.1999","article-title":"Characterization of human Mycobacterium bovis Bacillus Calmette\u2010Guerin\u2010reactive CD8+ T cells","volume":"67","author":"Smith SM","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_6_27_2","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.1128\/IAI.67.8.3980-3988.1999","article-title":"Early emergence of CD8+ T cells primed for production of type 1 cytokines in the lungs of Mycobacterium tuberculosis\u2013infected mice","volume":"67","author":"Serbina NV","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/11.Supplement_2.S353"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.12013"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180110"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(88)91292-3"},{"key":"e_1_2_6_32_2","first-page":"27","article-title":"Increased IL\u20104 production in response to virulent Mycobacterium tuberculosis in tuberculosis patients with advanced disease","volume":"18","author":"Ordway DJ","year":"2005","journal-title":"Acta Med Port"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(03)00043-9"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3146-3154.2003"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.001045"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3083.2012.02726.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3083.2012.02726.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T19:17:11Z","timestamp":1697311031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3083.2012.02726.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,22]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1111\/j.1365-3083.2012.02726.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3083.2012.02726.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,22]]},"assertion":[{"value":"2012-08-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}