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Here, we show that either constitutive or conditional enhanced expression of <jats:italic>ADR1<\/jats:italic> conferred significant drought tolerance. This was not a general feature of defence\u2010related mutants because <jats:italic>cir<\/jats:italic> (constitutive induced resistance)<jats:italic>1<\/jats:italic>, <jats:italic>cir2<\/jats:italic> and <jats:italic>cpr<\/jats:italic> (constitutive expressor of <jats:italic>PR<\/jats:italic> genes)<jats:italic>1<\/jats:italic>, which constitutively express systemic acquired resistance (SAR), failed to exhibit this phenotype. Cross\u2010tolerance was not a characteristic of <jats:italic>adr1<\/jats:italic> plants, rather they showed increased sensitivity to thermal and salinity stress. Hence, <jats:italic>adr1<\/jats:italic>\u2010activated signalling may antagonise some stress responses. Northern analysis of abiotic marker genes revealed that dehydration\u2010responsive element (<jats:italic>DRE<\/jats:italic>)<jats:italic>B2A<\/jats:italic> but not <jats:italic>DREB1A<\/jats:italic>, <jats:italic>RD<\/jats:italic> (response to dehydration)<jats:italic>29A<\/jats:italic> or <jats:italic>RD22<\/jats:italic> was expressed in <jats:italic>adr1<\/jats:italic> plant lines. Furthermore, <jats:italic>DREB2A<\/jats:italic> expression was salicylic acid (SA) dependent but NPR (non\u2010expressor of <jats:italic>PR<\/jats:italic> genes)1 independent. In <jats:italic>adr1<\/jats:italic>\/<jats:italic>ADR1 nahG<\/jats:italic> (naphthalene hydroxylase G), <jats:italic>adr1<\/jats:italic>\/<jats:italic>ADR1 eds<\/jats:italic> (enhanced disease susceptibility)<jats:italic>1<\/jats:italic> and <jats:italic>adr1<\/jats:italic>\/<jats:italic>ADR1 abi1<\/jats:italic> double mutants, drought tolerance was significantly reduced. Microarray analyses of plants containing a conditional <jats:italic>adr1<\/jats:italic> allele demonstrated that a significant number of the upregulated genes had been previously implicated in responses to dehydration. Therefore, biotic and abiotic signalling pathways may share multiple nodes and their outputs may have significant functional overlap.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2004.02086.x","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T19:38:45Z","timestamp":1104349125000},"page":"810-822","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":242,"title":["Drought tolerance established by enhanced expression of the <i>CC\u2013NBS\u2013LRR<\/i> gene, <i>ADR1<\/i>, requires salicylic acid, EDS1 and ABI1"],"prefix":"10.1111","volume":"38","author":[{"given":"Andrea","family":"Chini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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