{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:35:00Z","timestamp":1791214500222,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T00:00:00Z","timestamp":1125014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Virus\u2010induced gene silencing (VIGS) is an attractive method for assaying gene function in species that are resistant to conventional genetic approaches. However, VIGS has been shown to be effective in only a few, closely related plant species. Tobacco rattle virus (TRV), a bipartite RNA virus, has a wide host range and so in principle could serve as an efficient vector for VIGS in a diverse array of plant species. Here we show that a vector based on TRV sequences is effective at silencing the endogenous\n                    <jats:italic>phytoene desaturase<\/jats:italic>\n                    (\n                    <jats:italic>PapsPDS<\/jats:italic>\n                    ) gene in\n                    <jats:italic>Papaver somniferum<\/jats:italic>\n                    (opium poppy). We show that this vector does not compromise the growth or reproduction of poppy and the plants did not display viral symptoms. The silencing of\n                    <jats:italic>PapsPDS<\/jats:italic>\n                    resulted in a significant reduction in\n                    <jats:italic>PapsPDS<\/jats:italic>\n                    mRNA and a concomitant photobleached phenotype. The ability to rapidly assay gene function in\n                    <jats:italic>P. somniferum<\/jats:italic>\n                    will be valuable in manipulation of the opiate pathway in this pharmaceutically important species. We suggest that our vacuum infiltration method used to deliver TRV\u2010based vectors into poppy is a promising approach for expanding VIGS to diverse angiosperm species in which traditional delivery methods fail to induce VIGS. Furthermore, these studies demonstrate the utility of TRV\u2010VIGS for probing gene function in a basal eudicot species that is phylogenetically distant from model plant species.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-313x.2005.02520.x","type":"journal-article","created":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T04:32:50Z","timestamp":1125030770000},"page":"334-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":168,"title":["Virus\u2010induced gene silencing is an effective tool for assaying gene function in the basal eudicot species\n                    <i>Papaver somniferum<\/i>\n                    (opium poppy)"],"prefix":"10.1111","volume":"44","author":[{"given":"Lena C.","family":"Hileman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sin\u00e9ad","family":"Drea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"de Martino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Litt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian F.","family":"Irish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,8,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1332"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02102.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1033"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/2992015"},{"key":"e_1_2_6_6_1","first-page":"323","article-title":"The potential of virus\u2010induced gene silencing for speeding up functional characterization of plant genes","volume":"3","author":"Benedito V.A.","year":"2004","journal-title":"Genet. 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