{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:12:10Z","timestamp":1791155530419,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T00:00:00Z","timestamp":1156896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Plum pox virus (PPV) is able to replicate in inoculated leaves of <jats:italic>Nicotiana tabacum<\/jats:italic>, but is defective in systemic movement in this host. However, PPV produces a systemic infection in transgenic tobacco expressing the silencing suppressor P1\/HC\u2010Pro from tobacco etch virus (TEV). In this work we show that PPV is able to move to upper non\u2010inoculated leaves of tobacco plants expressing bacterial salicylate hydroxylase (NahG) that degrades salicylic acid (SA). Replication and accumulation of PPV is higher in the locally infected leaves of plants deficient in SA or expressing TEV P1\/HC\u2010Pro silencing suppressor. Accumulation of viral derived small RNAs was reduced in the NahG transgenic plants, suggesting that SA might act as an enhancer of the RNA\u2010silencing antiviral defense in tobacco. Besides, expression of SA\u2010mediated defense transcripts, such as those of pathogenesis\u2010related (PR) proteins PR\u20101 and PR\u20102 or alternative oxidase\u20101, as well as that of the putative RNA\u2010dependent RNA polymerase NtRDR1, is induced in response to PPV infection, and the expression patterns of these defense transcripts are altered in the TEV P1\/HC\u2010Pro transgenic plants. Long\u2010distance movement of PPV is highly enhanced in NahG\u2003\u00d7\u2003P1\/HC\u2010Pro double\u2010transgenic plants and systemic symptoms in these plants reveal that the expression of an RNA\u2010silencing suppressor and the lack of SA produce additive but distinct effects. Our results suggest that SA might act as an enhancer of the RNA\u2010silencing antiviral defense in tobacco, and that silencing suppressors, such as P1\/HC\u2010Pro, also alter the SA\u2010mediated defense. Both an RNA\u2010silencing and an SA\u2010mediated defense mechanism could act together to limit PPV infection.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2006.02861.x","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T11:35:42Z","timestamp":1156937742000},"page":"217-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":150,"title":["Salicylic acid\u2010mediated and RNA\u2010silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobacco"],"prefix":"10.1111","volume":"48","author":[{"given":"Josefa M.","family":"Alamillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Sa\u00e9nz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan 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