{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:20:11Z","timestamp":1791332411679,"version":"4.3.1"},"reference-count":63,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,1,24]],"date-time":"2007-01-24T00:00:00Z","timestamp":1169596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In tobacco (<jats:italic>Nicotiana tabacum<\/jats:italic>), wounding causes rapid activation of two mitogen\u2010activated protein kinases (MAPKs), wound\u2010induced protein kinase (WIPK) and salicylic acid (SA)\u2010induced protein kinase (SIPK), and the subsequent accumulation of jasmonic acid (JA). Our previous studies suggested that activation of WIPK is required for the production of wound\u2010induced JA. However, the exact role of WIPK remains unresolved. We generated transgenic tobacco plants in which either<jats:italic>WIPK<\/jats:italic>or<jats:italic>SIPK<\/jats:italic>were silenced using RNA interference to define the roles of WIPK and SIPK in the wound response. In addition, transgenic tobacco plants were generated in which both<jats:italic>WIPK<\/jats:italic>and<jats:italic>SIPK<\/jats:italic>were silenced to examine the possibility that they have redundant roles. Wound\u2010induced JA production was reduced compared with non\u2010silenced plants in all of the<jats:italic>WIPK<\/jats:italic>\u2010,<jats:italic>SIPK<\/jats:italic>\u2010 and<jats:italic>WIPK\/SIPK<\/jats:italic>\u2010silenced plants. Transgenic plants over\u2010expressing<jats:italic>NtMKP1<\/jats:italic>, a gene encoding tobacco MAPK phosphatase, which inactivates WIPK and SIPK, also exhibited reduced JA production in response to wounding. In both<jats:italic>WIPK\/SIPK<\/jats:italic>\u2010silenced and<jats:italic>NtMKP1<\/jats:italic>\u2010over\u2010expressing plants, wounding resulted in an abnormal accumulation of both SA and transcripts for SA\u2010responsive genes. These results suggest that WIPK and SIPK play an important role in JA production in response to wounding, and that they function cooperatively to control SA biosynthesis.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2006.03003.x","type":"journal-article","created":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T02:06:24Z","timestamp":1169690784000},"page":"899-909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":158,"title":["The mitogen\u2010activated protein kinases WIPK and SIPK regulate the levels of jasmonic and salicylic acids in wounded tobacco plants"],"prefix":"10.1111","volume":"49","author":[{"given":"Shigemi","family":"Seo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinpei","family":"Katou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideharu","family":"Seto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Gomi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Ohashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,2,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250050082"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/3870372"},{"key":"e_1_2_6_4_1","first-page":"343","article-title":"Perspectives on wound healing in resistance to pathogens","volume":"27","author":"Bostock R.M.","year":"1989","journal-title":"Annu. 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