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A 1095\u2003bp cDNA from elicited <jats:italic>Ruta<\/jats:italic> cells was expressed in <jats:italic>Escherichia coli<\/jats:italic>, and shown to encode <jats:italic>S\u2010<\/jats:italic>adenosyl\u2010<jats:sc>l<\/jats:sc>\u2010methionine\u2010dependent anthranilate <jats:italic>N<\/jats:italic>\u2010methyltransferase. SDS\u2013PAGE of the purified enzyme revealed a mass of 40\u2003\u00b1\u20032\u2003kDa, corresponding to 40\u2003059\u2003Da for the translated polypeptide, whereas the catalytic activity was assigned to a homodimer. Alignments revealed closest relationships to catechol or caffeate <jats:italic>O<\/jats:italic>\u2010methyltransferases at 56% and 55% identity (73% similarity), respectively, with little similarity (\u223c20%) to <jats:italic>N<\/jats:italic>\u2010methyltransferases for purines, putrescine, glycine, or nicotinic acid substrates. Notably, a single Asn residue replacing Glu that is conserved in caffeate <jats:italic>O<\/jats:italic>\u2010methyltransferases determines the catalytic efficiency. The recombinant enzyme showed narrow specificity for anthranilate, and did not methylate catechol, salicylate, caffeate, or 3\u2010 and 4\u2010aminobenzoate. Moreover, anthraniloyl CoA was not accepted. As <jats:italic>Ruta graveolens<\/jats:italic> acridone synthase also does not accept anthraniloyl CoA as a starter substrate, the anthranilate <jats:italic>N<\/jats:italic>\u2010methylation prior to CoA activation is a key step in acridone alkaloid formation, channelling anthranilate from primary into secondary branch pathways, and holds promise for biotechnological applications. RT\u2010PCR amplifications and Western blotting revealed expression of the <jats:italic>N<\/jats:italic>\u2010methyltransferase in all organs of <jats:italic>Ruta<\/jats:italic> plants, particularly in the flower and root, mainly associated with vascular tissues. This expression correlated with the pattern reported previously for expression of acridone synthase and acridone alkaloid accumulation.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2007.03360.x","type":"journal-article","created":{"date-parts":[[2008,1,25]],"date-time":"2008-01-25T10:49:49Z","timestamp":1201258189000},"page":"541-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Anthranilate <i>N<\/i>\u2010methyltransferase, a branch\u2010point enzyme of acridone biosynthesis"],"prefix":"10.1111","volume":"53","author":[{"given":"Britta","family":"Rohde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Hans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Martens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred","family":"Baumert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hunziker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Matern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,11,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.02.016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.07.005"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00269190"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-969929"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2800040406"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5574"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.2.507"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2005.05.026"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02680.x"},{"key":"e_1_2_6_11_1","first-page":"1151","article-title":"Chemical repellents and plastic netting for reducing bird damage to sweet cherries, blueberries and grapes","volume":"29","author":"Curtis P.D.","year":"1994","journal-title":"Hort. 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