{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:05:42Z","timestamp":1787727942744,"version":"build-2784847793"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,12,30]],"date-time":"2010-12-30T00:00:00Z","timestamp":1293667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>Triticum aestivum<\/jats:italic> (bread wheat) disease resistance gene <jats:italic>Lr34<\/jats:italic> confers durable, race non\u2010specific protection against three fungal pathogens, and has been a highly relevant gene for wheat breeding since the green revolution. <jats:italic>Lr34<\/jats:italic>, located on chromosome\u20037D, encodes an ATP\u2010binding cassette (ABC) transporter. Both wheat cultivars with and without <jats:italic>Lr34<\/jats:italic>\u2010based resistance encode a putatively functional protein that differ by only two amino acid polymorphisms. In this study, we focused on the identification and characterization of homoeologous and orthologous <jats:italic>Lr34<\/jats:italic> genes in hexaploid wheat and other grasses. In hexaploid wheat we found an expressed and putatively functional <jats:italic>Lr34<\/jats:italic> homoeolog located on chromosome\u20034A, designated <jats:italic>Lr34\u2010B<\/jats:italic>. Another homoeologous <jats:italic>Lr34<\/jats:italic> copy, located on chromosome\u20037A, was disrupted by the insertion of repetitive elements. Protein sequences of LR34\u2010B and LR34 were 97% identical. Orthologous <jats:italic>Lr34<\/jats:italic> genes were detected in the genomes of <jats:italic>Oryza sativa<\/jats:italic> (rice) and <jats:italic>Sorghum bicolor<\/jats:italic> (sorghum). <jats:italic>Zea mays<\/jats:italic> (maize), <jats:italic>Brachypodium distachyon<\/jats:italic> and <jats:italic>Hordeum vulgare<\/jats:italic> (barley) lacked <jats:italic>Lr34<\/jats:italic> orthologs, indicating independent deletion of this particular ABC transporter. <jats:italic>Lr34<\/jats:italic> was part of a gene\u2010rich island on the wheat\u2003D genome. We found gene colinearity on the homoeologous\u2003A and B genomes of hexaploid wheat, but little microcolinearity in other grasses. The homoeologous LR34\u2010B protein and the orthologs from rice and sorghum have the susceptible haplotype for the two critical polymorphisms distinguishing the LR34 proteins from susceptible and resistant wheat cultivars. We conclude that the particular <jats:italic>Lr34<\/jats:italic>\u2010haplotype found in resistant wheat cultivars is unique. It probably resulted from functional gene diversification that occurred after the polyploidization event that was at the origin of cultivated bread wheat.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2010.04430.x","type":"journal-article","created":{"date-parts":[[2010,11,19]],"date-time":"2010-11-19T15:46:24Z","timestamp":1290181584000},"page":"392-403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["<i>Lr34<\/i> multi\u2010pathogen resistance ABC transporter: molecular analysis of homoeologous and orthologous genes in hexaploid wheat and other grass species"],"prefix":"10.1111","volume":"65","author":[{"given":"Simon G.","family":"Krattinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evans S.","family":"Lagudah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wicker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna M.","family":"Risk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony R.","family":"Ashton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liselotte L.","family":"Selter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"Keller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1934431100"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF03543362","article-title":"Construction and characterisation of a hexaploid wheat (Triticum aestivum L.) BAC library from the reference germplasm \u2018Chinese Spring\u2019","volume":"31","author":"Allouis S.","year":"2003","journal-title":"Cereal Res. Commun"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02991.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02841.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81912-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803981105"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.029181"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.12.043"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-010-1316-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220890"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220051061"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166289"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175550"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.060756"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.4.378"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.11.5937"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03895"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.102.014720"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672305007391"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2005.05.001"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2007.08.0474"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2009.12.010"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166453"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-009-1097-z"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00222914"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.2007.00036.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1139\/g87-149"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1139\/g03-067"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl976"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-003-1361-6"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07723"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026353225758"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2008.10.006"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.096941"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-003-1567-7"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.57.032905.105406"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-12-227620-5\/00113-0"},{"key":"e_1_2_6_41_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2001"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178534"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2004.94.10.1036"},{"key":"e_1_2_6_44_1","first-page":"1","article-title":"The aneuploids of common wheat","volume":"572","author":"Sears S.R.","year":"1954","journal-title":"Mo. Aes. Res. Bull."},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-009-9959-7"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-004-1871-x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1101\/gr.1113003","article-title":"Comparative DNA sequence analysis of wheat and rice genomes","volume":"13","author":"Sorrells M.E.","year":"2003","journal-title":"Genome Res"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.038372"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.113004.133839"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08747"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2006.07.0489tpg"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220050906"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(02)02372-5"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.129734"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000409"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-313X.2010.04430.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-313X.2010.04430.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T03:10:06Z","timestamp":1698721806000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-313X.2010.04430.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,30]]},"references-count":54,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1111\/j.1365-313X.2010.04430.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-313x.2010.04430.x","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,30]]},"assertion":[{"value":"2010-12-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}