{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T06:46:56Z","timestamp":1793947616137,"version":"4.8.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Copper is an essential micronutrient that functions as a redox cofactor in multiple plant processes, including photosynthesis. <jats:italic>Arabidopsis thaliana<\/jats:italic> possesses a conserved family of CTR\u2010like high\u2010affinity copper transport proteins denoted as COPT1\u20105. COPT1, the only family member that is functionally characterized, participates in plant copper acquisition. However, little is known about the function of the other Arabidopsis COPT proteins in the transport and distribution of copper. Here, we show that a functional fusion of COPT5 to the green fluorescent protein localizes in Arabidopsis cells to the prevacuolar compartment. Plants defective in <jats:italic>COPT5<\/jats:italic> do not exhibit any significant phenotype under copper\u2010sufficient conditions, but their growth is compromised under copper limitation. Under extreme copper deficiency, two independent <jats:italic>copt5<\/jats:italic> knockout mutant lines exhibit severe defects in vegetative growth and root elongation, low chlorophyll content, and impairment in the photosynthetic electron transfer. All these phenotypes are rescued when the wild\u2010type copy of the <jats:italic>COPT5<\/jats:italic> gene is retransformed into a <jats:italic>copt5<\/jats:italic> knockout line or when copper, but not other metals, are added to the medium. <jats:italic>COPT5<\/jats:italic> is expressed in vascular tissues, with elevated levels in roots. Taken together, these results suggest that COPT5 plays an important role in the plant response to environmental copper scarcity, probably by remobilizing copper from prevacuolar vesicles, which could act as internal stores or recycling vesicles to provide the metal cofactor to key copper\u2010dependent processes such as photosynthesis.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2010.04472.x","type":"journal-article","created":{"date-parts":[[2010,12,24]],"date-time":"2010-12-24T15:14:19Z","timestamp":1293203659000},"page":"848-860","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":105,"title":["The intracellular Arabidopsis COPT5 transport protein is required for photosynthetic electron transport under severe copper deficiency"],"prefix":"10.1111","volume":"65","author":[{"given":"Antoni","family":"Garcia\u2010Molina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Andr\u00e9s\u2010Col\u00e1s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Perea\u2010Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Secundino","family":"del Valle\u2010Tasc\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lola","family":"Pe\u00f1arrubia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergi","family":"Puig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.030452"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086391"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.153676"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02601.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.05.001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206444200"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eri050"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2009.02846.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810286106"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203652200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2190001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.1.128"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(00)00065-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/41.5.548"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90358-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600250-MCP200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.47.28479"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.72"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20021128"},{"key":"e_1_2_6_22_1","first-page":"27","article-title":"Complementary PSII quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and saturation pulse method","volume":"1","author":"Klughammer C.","year":"2008","journal-title":"PAM Application Notes"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00719.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104728200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2003.12.003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/51.345.659"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.04.007"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207477200"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1962.tb08052.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R000006200"},{"key":"e_1_2_6_31_1","first-page":"105","article-title":"Discussion of spectrophotometric determination of marine\u2010plant pigments, with revised equations for ascertaining chlorophyll\u2010a and carotenoids","volume":"21","author":"Parsons T.","year":"1963","journal-title":"J. Mar. Res."},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-009-0591-8"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(02)00298-3"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202547200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2007.01642.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703397200"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411669200"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.118.2.691"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2005.01496.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.1.1-13.2004"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022345507112"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313321200"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.124.4.1477"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.011817"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.060137"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-313X.2010.04472.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-313X.2010.04472.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T19:40:52Z","timestamp":1698781252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-313X.2010.04472.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":44,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1111\/j.1365-313X.2010.04472.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-313x.2010.04472.x","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"2011-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}