{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:21:30Z","timestamp":1792074090803,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,11,29]],"date-time":"2011-11-29T00:00:00Z","timestamp":1322524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"Os03g0755100\">Os03g0755100<\/jats:ext-link> (<jats:italic>OsALS1<\/jats:italic>), that is regulated by ART1, a C2H2\u2010type zinc finger transcription factor. <jats:italic>OsALS1<\/jats:italic> encodes a half\u2010size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub\u2010group. Expression of <jats:italic>OsALS1<\/jats:italic> was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene <jats:italic>AtALS1<\/jats:italic> in Arabidopsis. Knockout of <jats:italic>OsALS1<\/jats:italic> in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild\u2010type and <jats:italic>osals1<\/jats:italic> mutants showed that there was no difference in Al levels in the cell sap of root tips between wild\u2010type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild\u2010type. Expression of <jats:italic>OsALS1<\/jats:italic> in yeast resulted in increased Al sensitivity due to mis\u2010localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2011.04837.x","type":"journal-article","created":{"date-parts":[[2011,10,29]],"date-time":"2011-10-29T16:52:45Z","timestamp":1319907165000},"page":"857-867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":285,"title":["A tonoplast\u2010localized half\u2010size ABC transporter is required for internal detoxification of aluminum in rice"],"prefix":"10.1111","volume":"69","author":[{"given":"Chao\u2010Feng","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Yamaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Feng","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1991.tb00121.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2006.00346.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.3.695"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eri136"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm091"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.5511\/plantbiotechnology.18.219"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602868103"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.064543"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00192809"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01319-1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2006.01509.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.46.060195.001321"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141655"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0452-4"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.3.1107"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.085233"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2008.03696.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/41.4.383"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(07)64005-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/37518"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.4.1033"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.117.3.753"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(01)01961-6"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh152"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch060"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pci116"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng2074"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.04103.x"},{"key":"e_1_2_8_30_1","first-page":"1","volume-title":"Metal Ions in Biological Systems. 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