{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:19:49Z","timestamp":1791346789398,"version":"4.3.1"},"reference-count":74,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T00:00:00Z","timestamp":1331164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Plant development and function are underpinned by redox reactions that depend on co\u2010factors such as nicotinamide adenine dinucleotide (NAD). NAD has recently been shown to be involved in several signalling pathways that are associated with stress tolerance or defence responses. However, the mechanisms by which NAD influences plant gene regulation, metabolism and physiology still remain unclear. Here, we took advantage of <jats:italic>Arabidopsis thaliana<\/jats:italic> lines that overexpressed the <jats:italic>nadC<\/jats:italic> gene from <jats:italic>E.\u00a0coli<\/jats:italic>, which encodes the NAD biosynthesis enzyme quinolinate phosphoribosyltransferase (QPT). Upon incubation with quinolinate, these lines accumulated NAD and were thus used as inducible systems to determine the consequences of an increased NAD content in leaves. Metabolic profiling showed clear changes in several metabolites such as aspartate\u2010derived amino acids and NAD\u2010derived nicotinic acid. Large\u2010scale transcriptomic analyses indicated that NAD promoted the induction of various pathogen\u2010related genes such as the salicylic acid (SA)\u2010responsive defence marker <jats:italic>PR1.<\/jats:italic> Extensive comparison with transcriptomic databases further showed that gene expression under high NAD content was similar to that obtained under biotic stress, eliciting conditions or SA treatment. Upon inoculation with the avirulent strain of <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato Pst\u2010AvrRpm1<\/jats:italic>, the <jats:italic>nadC<\/jats:italic> lines showed enhanced resistance to bacteria infection and exhibited an <jats:italic>ICS1<\/jats:italic>\u2010dependent build\u2010up of both conjugated and free SA pools. We therefore concluded that higher NAD contents are beneficial for plant immunity by stimulating SA\u2010dependent signalling and pathogen resistance.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2012.04920.x","type":"journal-article","created":{"date-parts":[[2012,1,23]],"date-time":"2012-01-23T12:45:28Z","timestamp":1327322728000},"page":"650-665","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Inducible NAD overproduction in Arabidopsis alters metabolic pools and gene expression correlated with increased salicylate content and resistance to <i>Pst\u2010AvrRpm1<\/i>"],"prefix":"10.1111","volume":"70","author":[{"given":"Pierre","family":"P\u00e9triacq","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"de Bont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutta","family":"Hager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Didierlaurent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Mauve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Gu\u00e9rard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Noctor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Pelletier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Renou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Tcherkez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Gaki\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.04.034"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.148049"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.50.1.601"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2005.03.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.092569"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4198"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2004.01.007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.133348"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.073651"},{"key":"e_1_2_8_11_1","first-page":"207","article-title":"Feedback inhibition of dihydrodipicolinate synthase enzymes by L\u2013lysine","volume":"378","author":"Blicking S.","year":"1997","journal-title":"Biol. 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