{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:51:48Z","timestamp":1792666308397,"version":"4.5.0"},"reference-count":71,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T00:00:00Z","timestamp":1350000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The Golgi\u2010located phosphate exporter PHT4;6 has been described as involved in salt tolerance but further analysis on the physiological impact of PHT4;6 remained elusive. Here we show that PHT4;6\u2013GFP is targeted to the <jats:italic>trans<\/jats:italic>\u2010Golgi compartment and that loss of function of this carrier protein has a dramatic impact on plant growth and development. Knockout mutants of <jats:italic>pht4;6<\/jats:italic> exhibit a dwarf phenotype that is complemented by the homologous gene from rice (<jats:italic>Oryza sativa<\/jats:italic>). Interestingly, <jats:italic>pht4;6<\/jats:italic> mutants show altered characteristics of several Golgi\u2010related functions, such as an altered abundance of certain N\u2010glycosylated proteins, altered composition of cell\u2010wall hemicelluose, and higher sensitivity to the Golgi \u03b1\u2010mannosidase and the retrograde transport inhibitors kifunensine and brefeldin\u2003A, respectively. Moreover, <jats:italic>pht4;6<\/jats:italic> mutants exhibit a \u2018mimic disease\u2019 phenotype accompanied by constitutively activated pathogen defense mechanisms and increased resistance against the virulent <jats:italic>Pseudomonas syringae<\/jats:italic> strain DC3000. Surprisingly, <jats:italic>pht4;6<\/jats:italic> mutants also exhibit phosphate starvation symptoms, as revealed at the morphological and molecular level, although total Pi levels in wild\u2010type and <jats:italic>pht4;6<\/jats:italic> plants are similar. This suggested that subcellular Pi compartmentation was impaired. By use of nuclear magnetic resonance (NMR), increased Pi concentration was detected in acidic compartments of <jats:italic>pht4;6<\/jats:italic> mutants. We propose that impaired Pi efflux from the <jats:italic>trans<\/jats:italic>\u2010Golgi lumen results in accumulation of inorganic phosphate in other internal compartments, leading to low cytoplasmic phosphate levels with detrimental effects on plant performance.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2012.05106.x","type":"journal-article","created":{"date-parts":[[2012,7,13]],"date-time":"2012-07-13T11:17:44Z","timestamp":1342178264000},"page":"732-744","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis"],"prefix":"10.1111","volume":"72","author":[{"given":"Sebastian","family":"Hassler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Lemke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"M\u00f6hlmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falco","family":"Kr\u00fcger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Schumacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Espen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Martinoia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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