{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T02:40:06Z","timestamp":1768704006668,"version":"3.49.0"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T00:00:00Z","timestamp":1158883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>summary<\/jats:sc><\/jats:bold> Culture methods for bacterial detection (BacT\/ALERT or Pall eBDS) are currently implemented in blood donor screening procedures in many countries. Experience in the first years after implementation of these detection assays showed that although the analytical sensitivity was extremely high (about 1 CFU mL<jats:sup>\u22121<\/jats:sup>), the majority of these platelets were still transfused before a positive screening result was attained. Rapid technologies were developed to more effectively prevent transfusion\u2010transmitted bacterial infection. In this study, a new rapid bacterial detection method based on fluorescence\u2010activated cell sorting (FACS) technology was developed. Bacteria were stained with thiazole orange dye for 5 min and measurement was taken immediately after staining. The entire process took only 30 min. Six transfusion\u2010relevant bacteria strains were tested in a spiking study. Without pre\u2010incubation in a special bacteria growth medium, analytical sensitivity ranged between 10<jats:sup>5<\/jats:sup> CFU mL<jats:sup>\u22121<\/jats:sup> (<jats:italic>Klebsiella oxytoca and Serratia marcesens<\/jats:italic>) and 10<jats:sup>3<\/jats:sup> CFU mL<jats:sup>\u22121<\/jats:sup> (<jats:italic>Escherichia coli<\/jats:italic>). Sensitivity could be improved to 10<jats:sup>1<\/jats:sup> CFU mL<jats:sup>\u22121<\/jats:sup> for all tested bacteria by adding a pre\u2010incubation step (6 h at 37 \u00b0C). Although preliminary in nature, results of our study suggest that bacterial detection by FACS technology in conjunction with a pre\u2010incubation step offers a sensitive alternative technology to culture methods. Additionally, it provides the benefit of a rapid test time and the opportunity of preventing bacterial transmitted infections more effectively.<\/jats:p>","DOI":"10.1111\/j.1365-3148.2006.00686.x","type":"journal-article","created":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T12:59:12Z","timestamp":1158929952000},"page":"355-361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["FACS technology used in a new rapid bacterial detection method"],"prefix":"10.1111","volume":"16","author":[{"given":"M.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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