{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:40:41Z","timestamp":1785415241334,"version":"3.56.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,4,8]],"date-time":"2008-04-08T00:00:00Z","timestamp":1207612800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p><jats:bold><jats:sc>summary<\/jats:sc><\/jats:bold> We present here our overall experience after 27\u2003months of performance of the Procleix Ultrio [HIV\u20101, hepatitis C virus (HCV), hepatitis B virus (HBV)] transcription\u2010mediated amplification (TMA) assay.<\/jats:p><jats:p>The aim of this report is to assess the impact of nucleic acid testing (NAT) implementation in blood screening of south\u2010western Greek blood donors.<\/jats:p><jats:p>We processed 38\u2003264\u2003units of blood as neat samples with the Procleix Ultrio TMA assay (Chiron\/GenProbe, Emeryville\/San Diego, CA, USA) between 1 January 2005 and 31 March 2007. NAT results were compared to those obtained from routine serology tests and quantitative polymerase chain reaction (PCR) assays.<\/jats:p><jats:p>Overall, 52\u2003units of blood tested positive for HBV (1\u00b74\u2030), 8 for HCV (0\u00b72\u2030) and none for HIV or multiple infections. The yield of TMA was 0\u00b7183\u2030 for HBV (7\/38\u2003264) and 0 for HCV. The TMA HBV\u2010positive donations were tested for HBV DNA by a quantitative PCR assay and were found negative (below the detection limit of the method, 200 copies\/mL). Follow\u2010up testing showed that the TMA HBV\u2010positive donations were positive for anti\u2010hepatitis B core antigen immunoglobulin G antibodies.<\/jats:p><jats:p>Implementation of the TMA assay in the individual donation configuration increased HBV detection compared to serological screening or a commonly used quantitative PCR assay. Follow\u2010up studies will determine the impact of NAT implementation in HBV transmission in countries with an intermediate HBV incidence.<\/jats:p>","DOI":"10.1111\/j.1365-3148.2008.00847.x","type":"journal-article","created":{"date-parts":[[2008,4,8]],"date-time":"2008-04-08T08:27:42Z","timestamp":1207643262000},"page":"104-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Procleix Ultrio transcription\u2010mediated amplification vs. serological blood screening in south\u2010western Greece"],"prefix":"10.1111","volume":"18","author":[{"given":"A.","family":"Sgourou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Karakantza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Theodori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Papadimitriou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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C.","family":"Zoumbos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mouzaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.2000.00265.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0042-9007.2004.00406.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00424.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21413"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00649.x"},{"key":"e_1_2_6_7_1","unstructured":"Chudy M. Amberg I. Hanker\u2010Ducel C.&N\u00fcbling C.M. 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