{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:57:25Z","timestamp":1789181845204,"version":"build-2803163510"},"reference-count":10,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T00:00:00Z","timestamp":1081814400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We conducted a sero\u2010epidemiological study of kala\u2010azar in two endemic communities (Kasaini and Gidhaniya) situated in the Terai (plain) of eastern Nepal. Direct agglutination test (DAT) was used as a serological test for screening. Capillary blood samples were collected by filter paper method from 601 (96%) people of a total population of 628 in Kasaini and from 482 (94%) people of 515 in Gidhaniya. Positive DAT titres (1:2000) were found in 66 (6.09%) of 1083 sera tested. The male\u2013female sero\u2010prevalence ratio was 1.44:1 and the age group of 15\u2003years and above was most affected. Among the bone marrow aspirates collected from 66 DAT seropositive cases, only 19 were positive for <jats:italic>Leishmania donovani<\/jats:italic> (LD bodies). Of the 47 DAT seropositive but LD bodies\u2019 negative cases, three were clinically active cases of kala\u2010azar. Another nine developed clinical symptoms of kala\u2010azar during 6\u2003months follow\u2010up and 23 were cases that had received prior treatment for kala\u2010azar (within 1\u2003year). The results of this study show the potential of the DAT on filter paper as a screening test for the surveillance of kala\u2010azar at a community level.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2004.01212.x","type":"journal-article","created":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T08:05:46Z","timestamp":1081843546000},"page":"533-537","source":"Crossref","is-referenced-by-count":23,"title":["Epidemiological study of kala\u2010azar by direct agglutination test in two rural communities of eastern Nepal"],"prefix":"10.1111","volume":"9","author":[{"given":"S.","family":"Koirala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Karki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L.","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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