{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:06:26Z","timestamp":1761987986996},"reference-count":5,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,4,29]],"date-time":"2004-04-29T00:00:00Z","timestamp":1083196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>High prevalences of intestinal amoebiasis are commonly reported by microscopy in Ethiopia. In order to confirm the actual occurrence of <jats:italic>Entamoeba histolytica<\/jats:italic> we collected 108 stool specimens from different hospitals &amp; health centers from patients in whom haematophagous trophozoites were believed to be found. We detected only a single <jats:italic>E. histolytica<\/jats:italic> case while 77 (71.3%) were <jats:italic>E. dispar<\/jats:italic> and the remaining 30 samples were negative for both species by real\u2010time PCR based on the small subunit ribosomal RNA gene sequence of <jats:italic>E. histolytica<\/jats:italic> and <jats:italic>E. dispar<\/jats:italic>. The tradition of microscopy in a routine diagnostic set\u2010up appears unsatisfactory to reliably differentiate rbc\u2010engulfing amoeba from non\u2010invasive amoeba in wet smears.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2004.01236.x","type":"journal-article","created":{"date-parts":[[2004,4,29]],"date-time":"2004-04-29T10:13:44Z","timestamp":1083233624000},"page":"651-652","source":"Crossref","is-referenced-by-count":36,"title":["Short communication: Misleading microscopy in amoebiasis"],"prefix":"10.1111","volume":"9","author":[{"given":"Amha","family":"Kebede","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaco J.","family":"Verweij","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beyene","family":"Petros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton M.","family":"Polderman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,29]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.236"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(03)90153-2"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1179\/000349804225003082"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1360-2276.2003.01145.x"},{"key":"e_1_2_2_6_1","first-page":"97","article-title":"Amoebiasis","volume":"72","author":"World Health Organization (WHO)","year":"1997","journal-title":"WHO Weekly Epidemiological Record"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3156.2004.01236.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3156.2004.01236.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T14:54:12Z","timestamp":1696949652000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3156.2004.01236.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,29]]},"references-count":5,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1111\/j.1365-3156.2004.01236.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3156.2004.01236.x","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,29]]}}}