{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:41:28Z","timestamp":1762270888600},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>objective<\/jats:bold> To evaluate the clinical value of flow cytometry anti\u2010live promastigate antibody (FC\u2010ALPA), for diagnosing active cutaneous leishmaniasis.<\/jats:p><jats:p><jats:bold>method<\/jats:bold> Serum samples from 145 individuals living in endemic areas for localized cutaneous leishmaniasis (population 1) were classified as having the disease or not and then tested for their IgG reactivity by indirect immunofluorescence assay and FC\u2010ALPA\u2010IgG. The results of FC\u2010ALPA\u2010IgG were expressed as percentage of positive fluorescent parasite. Both tests were also evaluated in serum samples of people with visceral leishmaniasis and Chagas disease (population 1A).<\/jats:p><jats:p><jats:bold>results<\/jats:bold> In population 1, FC\u2010ALPA\u2010IgG performed better than the immunofluorescence assay regarding sensitivity, specificity and predictive values. Analysis of the results according to the likelihood ratios indicated that a percentage of positive fluorescent parasite \u226460 practically excludes the diagnosis of localized cutaneous leishmaniasis (likelihood ratio\u2003=\u20030.07), whereas at &gt;60% it reinforces diagnosis of the disease (likelihood ratio\u2003=\u20037.0). Immunofluorescent assay is of little value (likelihood ratio\u2003=\u20032.04). In population 1A, both tests performed worse, but FC\u2010ALPA\u2010IgG achieved better statistical indexes than immunofluorescent assay.<\/jats:p><jats:p><jats:bold>conclusion<\/jats:bold> The FC\u2010ALPA\u2010IgG is a valuable method for serological diagnosis of localized cutaneous leishmaniasis. FC\u2010ALPA\u2010IgG1\/ALPA\u2010IgG2 combined analysis is an additional serological tool for discriminating localized visceral leishmaniasis, Chagas disease and visceral leishmaniasis in areas where these infections co\u2010exist.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2005.01552.x","type":"journal-article","created":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T14:41:34Z","timestamp":1138804894000},"page":"156-166","source":"Crossref","is-referenced-by-count":22,"title":["Clinical value of anti\u2010live <i>Leishmania (Viannia) braziliensis<\/i> immunoglobulin G subclasses, detected by flow cytometry, for diagnosing active localized cutaneous leishmaniasis"],"prefix":"10.1111","volume":"11","author":[{"given":"R. D. R.","family":"Rocha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M. F.","family":"Gontijo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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