{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:33:05Z","timestamp":1791217985230,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T00:00:00Z","timestamp":1140652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold> To investigate the role of <jats:italic>Anopheles subpictus<\/jats:italic> Grassi as a vector of Japanese encephalitis virus (JEV) transmission in Cuddalore, an area of Tamil Nadu endemic for the disease.<\/jats:p><jats:p><jats:bold>Method\u2002<\/jats:bold> We collected 98 pools (4900 specimens) of wild adult male <jats:italic>An. subpictus<\/jats:italic> mosquitoes outdoors during dusk hours and screened them for JEV antigen by antigen\u2010capture Enzyme Linked Immunosorbent Assay. Additionally, over a period of 1\u2003year, we tested 166 pools (8300 specimens) of wild adult female <jats:italic>An. subpictus<\/jats:italic> mosquitoes collected indoors for JEV.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Four pools of male <jats:italic>An. subpictus<\/jats:italic> tested positive. This indicates possible natural transovarial transmission of the virus through <jats:italic>An. subpictus<\/jats:italic>. Nineteen female pools were positive with a minimum infection rate of 2.3. From January through March the maximum infection rate was highest: 5.0 compared with 1.7 between April and September and 2.1 from October to December, although the difference was not statistically significant. From the 19 positive female pools, four isolates were confirmed as JEV by insect bioassay.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> The role of <jats:italic>An. subpictus<\/jats:italic> as a secondary vector in JEV transmission in Cuddalore, Tamil Nadu lends support to the hypothesis of periodic epidemics in the region.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2006.01567.x","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T16:51:58Z","timestamp":1140713518000},"page":"288-293","source":"Crossref","is-referenced-by-count":32,"title":["Long\u2010term study of Japanese encephalitis virus infection in <i>Anopheles subpictus<\/i> in Cuddalore district, Tamil Nadu, South India"],"prefix":"10.1111","volume":"11","author":[{"given":"V.","family":"Thenmozhi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Rajendran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ayanar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Manavalan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. K.","family":"Tyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,23]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"124","article-title":"Multiple host feeding in field populations of Anopheles culicifacies and An. subpictus in Sri Lanka. Medical and Veterinary","volume":"13","author":"Amerasinghe PH","year":"1999","journal-title":"Entomology"},{"key":"e_1_2_6_3_1","first-page":"10","volume-title":"Annual Report","author":"Centre for Research in Medical Entomology","year":"1997"},{"key":"e_1_2_6_4_1","first-page":"77","article-title":"The first epidemic of Japanese encephalitis studied in India \u2013 virological studies","volume":"63","author":"Chakravarty SK","year":"1975","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_6_5_1","first-page":"759","article-title":"Mosquito vectors of Japanese encephalitis virus and their bionomics in India","volume":"46","author":"Dhanda V","year":"1980","journal-title":"Proceedings of Indian National Sciency Academy"},{"key":"e_1_2_6_6_1","first-page":"4","article-title":"Virus isolation from wild\u2010caught mosquitoes during a Japanese encephalitis outbreak in Kerala in 1996","volume":"106","author":"Dhanda V","year":"1997","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_6_7_1","first-page":"89","article-title":"Mosquito transmitted flavivirus infections in India","volume":"1","author":"Gajanana A","year":"1998","journal-title":"Advanced Medical Entomology and Human Welfare UPZS (Suppl.)"},{"key":"e_1_2_6_8_1","first-page":"91","article-title":"Comparative evaluation of bioassay and ELISA for detection of Japanese encephalitis virus in field collected mosquitoes","volume":"26","author":"Gajanana A","year":"1995","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/34.6.651"},{"key":"e_1_2_6_10_1","first-page":"235","article-title":"Isolation of Japanese encephalitis and West Nile viruses from mosquitoes collected in Kolar district of Karnataka state during 1977\u201379","volume":"85","author":"George S","year":"1987","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_6_11_1","first-page":"399","article-title":"A study on vectors of Bancroftian filariasis in West Flores, Indonesia","volume":"11","author":"Hoedojo F","year":"1980","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"e_1_2_6_12_1","first-page":"104","article-title":"A long\u2010term study on vector abundance & seasonal prevalence in relation to the occurrence of Japanese encephalitis in Gorakhpur district, Uttar Pradesh","volume":"117","author":"Kanojia PC","year":"2003","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_6_13_1","first-page":"30","article-title":"Mosquito breeding in relation to aquatic vegetation and some physico\u2010chemical parameters in rice fields of central Gujarat","volume":"33","author":"Kant R","year":"1996","journal-title":"Indian Journal of Malariology"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/jvi.45.1.124-132.1983","article-title":"Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies","volume":"45","author":"Kimura\u2010Kuroda J","year":"1983","journal-title":"Journal of Virology"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(89)90288-5"},{"key":"e_1_2_6_16_1","first-page":"72","article-title":"Role of wastewater irrigation in mosquito breeding in south Punjab, Pakistan","volume":"34","author":"Mukhtar M","year":"2003","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"e_1_2_6_17_1","first-page":"290","article-title":"Seasonal prevalence of Culex vishnui subgroup, the major vectors of Japanese encephalitis virus in an endemic district of Andhra Pradesh, India","volume":"18","author":"Murty US","year":"2002","journal-title":"Journal of American Mosquito Control Association"},{"key":"e_1_2_6_18_1","first-page":"694","article-title":"Anopheles subpictus vector of malaria in coastal villages of south east India","volume":"50","author":"Panicker K.N.","year":"1981","journal-title":"Current Science"},{"key":"e_1_2_6_19_1","first-page":"37","article-title":"Japanese encephalitis virus infection in mosquitoes and its epidemiological implications","volume":"30","author":"Philip Samuel P","year":"2000","journal-title":"ICMR Bulletin"},{"key":"e_1_2_6_20_1","first-page":"520","article-title":"Anopheline mosquitoes of Malaya and North Borneo","volume":"31","author":"Reid JA","year":"1968","journal-title":"Studies from the Institute for Medical Research Malaysia"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/8.3.258"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02752458"},{"key":"e_1_2_6_23_1","first-page":"118","article-title":"Susceptibility status of Japanese encephalitis vectors in Kurnool and Mehboobnagar districts of Andhra Pradesh","volume":"35","author":"Sharma RS","year":"2003","journal-title":"Indian Journal of Communicable Diseases"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/jare.2000.0698"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3156.2006.01567.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3156.2006.01567.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T15:32:07Z","timestamp":1697297527000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3156.2006.01567.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,23]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1111\/j.1365-3156.2006.01567.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3156.2006.01567.x","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,23]]}}}